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B-Raf Proto-Oncogene, Serine/Threonine Kinase Peptide 5

Antibody: CPTC-BRAF-7
Background

Catalog Number:

CPTC-BRAF-7

RRID:

AB_2827860

Target Antigen:

B-Raf Proto-Oncogene, Serine/Threonine Kinase Peptide 5

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/26/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

Thematic Panel(s):

Ras Pathway

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Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR

CPTC-BRAF-7 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Negative

Affinity and binding kinetics of CPTC-BRAF-7 and full length BRAF recombinant protein were measured using surface plasmon resonance. CPTC-BRAF-7 rabbit antibody was captured on series S protein A biosensor chip. BRAF recombinant protein at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM, was used as analyte.


Characterization SOP Files

  IHC Tissue

CPTC-BRAF-7 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immunofluorescence

CPTC-BRAF-7 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC- BRAF-7 antibody. BRAF protein expression was not detected.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-BRAF-7 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-BRAF-7 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-BRAF-7 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-BRAF-7 as primary Ab against recombinant BRAF (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-BRAF-7 Western Blot

Result: Positive

Western Blot using CPTC-BRAF-7 as primary Ab against recombinant BRAF (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-BRAF-7 as primary Ab against MCF10A-KRas cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-BRAF-7 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-BRAF-7 as primary Ab against MCF10A-KRas cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: B-Raf Proto-Oncogene, Serine/Threonine Kinase Peptide 5
Background

NCI Identification Number:

00181

Antigen Name:

B-Raf Proto-Oncogene, Serine/Threonine Kinase Peptide 5

CPTC Name:

CPTC-BRAF Peptide 5

Aliases:

B-Raf Proto-Oncogene, Serine/Threonine Kinase; V-Raf Murine Sarcoma Viral Oncogene Homolog B1; V-Raf Murine Sarcoma Viral Oncogene Homolog B; Proto-Oncogene B-Raf; BRAF1; RAFB1; B-Raf Proto-Oncogene Serine/Threonine-Protein Kinase (P94); Murine Sarcoma Viral (V-Raf) Oncogene Homolog B1; Serine/Threonine-Protein Kinase B-Raf; 94 KDa B-Raf Protein; EC 2.7.11.1; B-RAF1; B-Raf; NS7; P94

Function:

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.

BRAF (B-Raf Proto-Oncogene, Serine/Threonine Kinase) is a Protein Coding gene. Diseases associated with BRAF include Cardiofaciocutaneous Syndrome and Lung Cancer. Among its related pathways are Development Slit-Robo signaling and MAPK Signaling: Oxidative Stress Pathway. GO annotations related to this gene include calcium ion binding and transferase activity, transferring phosphorus-containing groups. An important paralog of this gene is RAF1.

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Raf kinases, a family of three serine/threonine kinases, are part of the Ras-MAPK signaling cascade and phosphorylate MEK. Upon growth factor stimulation, Raf-1 (c-Raf) is activated by GTP-bound Ras and recruited to the cell membrane. This activation process is tightly regulated.

Chromosomal Localization:

7q34

Accession Number:

NP_004324.2

UniProt Accession Number:

P15056

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GDGGSTTGLSA(pT)PPASLPGSLTNVK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2750

Last Updated:

12/22/2016

Links

Characterization Data

SOPs

No SOPs available.

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