Background
Catalog Number:
CPTC-FEN1-1
RRID:
AB_2888954
Target Antigen:
Flap Structure-Specific Endonuclease 1 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
03/20/2024
Antigen Recognition(s):
Peptide, Endogenous
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
External Links
- CPTAC Assay Portal CPTAC-3230
- CPTAC Assay Portal CPTAC-700
Purchase
- DSHB CPTC-FEN1-1
Publications
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (327.6 KB)
CPTC-FEN1-1 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Immuno-MRM
CPTC-FEN1-1 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-FEN1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3230)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate. Data collected from plasma are available on the CPTAC assay portal (https://assays.cancer.gov/CPTAC-700)
Characterization SOP Files
Indirect ELISA
CPTC-FEN1-1
Result: Positive
Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
Background
NCI Identification Number:
00027
Antigen Name:
Flap Structure-Specific Endonuclease 1 Peptide 1
CPTC Name:
CPTC-FEN1 Peptide 1
Aliases:
FEN-1; MF1; RAD2; Maturation Factor 1; DNase IV; Flap Endonuclease 1; Maturation Factor-1; EC 3.1.; HFEN-1
Function:
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Chromosomal Localization:
11q12
Accession Number:
NP_004102.1
UniProt Accession Number:
P39748
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
SIEEIVR
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
770
Last Updated:
06/11/2013
Links
- EnsEMBL - EnsEMBL FEN1
- Entrez - Entrez FEN1
- Uniprot - Uniprot P39748
Characterization Data
SOPs
No SOPs available.
Don't have Adobe Reader™?
Get it for free at Adobe.com