Background
Catalog Number:
CPTC-ZFAND6-1
RRID:
AB_2617386
Target Antigen:
Zinc Finger, AN1-type Domain 6
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-ZFAND6-1
Characterization Data [Compare Characterization Data]
IHC HPA
CPTC-ZFAND6-1 Evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-ZFAND6-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-ZFAND6-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-ZFAND6-1 Western blot
Result: Positive
Western Blot using CPTC-ZFAND6-1 as primary Ab against ZFAND6 (rAg 00014) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-ZFAND6-2
RRID:
AB_2617387
Target Antigen:
Zinc Finger, AN1-type Domain 6
Isotype:
IgG2c
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-ZFAND6-2
Characterization Data [Compare Characterization Data]
IHC HPA
CPTC-ZFAND6-2 Evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-ZFAND6-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-ZFAND6-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-ZFAND6-2 Western blot
Result: Positive
Western Blot using CPTC-ZFAND6-2 as primary Ab against ZFAND6 (rAg 00014) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-ZFAND6-3
RRID:
AB_2617388
Target Antigen:
Zinc Finger, AN1-type Domain 6
Isotype:
IgG2c
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-ZFAND6-3
Characterization Data [Compare Characterization Data]
IHC HPA
CPTC-ZFAND6-3 Evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-ZFAND6-3 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-ZFAND6-3 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-ZFAND6-3 Western blot
Result: Positive
Western Blot using CPTC-ZFAND6-3 as primary Ab against ZFAND6 (rAg 00014) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
00014
Antigen Name:
Zinc Finger, AN1-type Domain 6
CPTC Name:
CPTC-ZFAND6
Aliases:
AWP1; ZA20D3; ZFAND5B; zinc finger, A20 domain containing 3; AN1-type zinc finger protein 6; protein associated with PRK1; Associated with PRK1 protein; Zinc finger A20 domain-containing protein 3
Function:
Involved in regulation of TNF-alpha induced NF-kappa-B activation and apoptosis. Involved in modulation of 'Lys-48'-linked polyubiquitination status of TRAF2 and decreases association of TRAF2 with RIPK1. Required for PTS1 target sequence-dependent protein import into peroxisomes and PEX5 stability; may cooperate with PEX6. In vitro involved in PEX5 export from the cytosol to peroxisomes.
Chromosomal Localization:
15q25.1
Expression System:
E.coli
Accession Number:
NM_019006
UniProt Accession Number:
Q6FIF0
DNA Source:
Imagenes
Immunogen:
Recombinant Full Length Protein
Vector Name:
pNIC28-Bsa4
Extinction Coefficient:
Buffers:
NaP
Expressed Sequence:
MHHHHHHSSGVDLGTENLYFQSMAQETNHSQVPMLCSTGCGFYGNPRTNG
MCSVCYKEHLQRQNSSNGRISPPATSVSSLSESLPVQCTDGSVPEAQSAL
DSTSSSMQPSPVSNQSLLSESVASSQLDSTSVDKAVPETEDVQASVSDTA
QQPSEEQSKPLEKPKQKKNRCFMCRKKVGLTGFECRCGNVYCGVHRYSDV
LNCSYNYKADAAEKIRKENPVVVGEKIQK
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
24981
Last Updated:
04/17/2013
Links
- EnsEMBL - EnsEMBL ZFAND6
- Entrez - Entrrez ZFAND6
- Uniprot - Uniprot Q6FIF0
Characterization Data
Gel
Click image to enlarge
CPTC-ZFAND6 PAGE
PAGE of ZFAND6 (rAg 00014) with molecular weight standards in lane 1.
Characterization SOP Files
SOPs
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