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X-Ray Repair Cross Complementing 5 Peptide 1

Antibody: CPTC-XRCC5-1
Background

Catalog Number:

CPTC-XRCC5-1

RRID:

AB_2889971

Target Antigen:

X-Ray Repair Cross Complementing 5 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Peptide

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (490.6 KB)

CPTC-XRCC5-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (490.6 KB)

CPTC-XRCC5-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CTPC-XRCC5-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate

CPTC-XRCC5-1 Western Blot (Over-Expression Cell Lysate)

Result: Negative

Western blot using CPTC-XRCC5-1 as primary antibody against XRCC5 overexpression cell lysate (lane 2). Expected molecular weight - 82.7 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: X-Ray Repair Cross Complementing 5 Peptide 1
Background

NCI Identification Number:

00402

Antigen Name:

X-Ray Repair Cross Complementing 5 Peptide 1

CPTC Name:

CPTC-XRCC5 Peptide 1

Aliases:

X-Ray Repair Cross Complementing 5; Ku86; X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5 (Double-Strand-Break Rejoining); ATP-Dependent DNA Helicase II 80 KDa Subunit; X-Ray Repair Cross-Complementing Protein 5; ATP-Dependent DNA Helicase 2 Subunit 2; CTC Box-Binding Factor 85 KDa Subunit; Lupus Ku Autoantigen Protein P86; 86 KDa Subunit Of Ku Antigen; Thyroid-Lupus Autoantigen; DNA Repair Protein XRCC5; Ku Autoantigen, 80kDa; Nuclear Factor IV; KARP-1; CTC85; CTCBF; KU80; KUB2; TLAA; X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 5 (Double-Strand-Break Rejoining; Ku Autoantigen, 80kD); Ku86 Autoantigen Related Protein 1; EC 3.6.4.-; KARP1; XRCC5; G22P2; NFIV; Ku80

Function:

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC5 (X-Ray Repair Cross Complementing 5) is a Protein Coding gene. Diseases associated with XRCC5 include Werner Syndrome and Ataxia-Telangiectasia. Among its related pathways are Telomere Extension by Telomerase and Cytosolic sensors of pathogen-associated DNA. Gene Ontology (GO) annotations related to this gene include transcription regulatory region DNA binding. An important paralog of this gene is XRCC6.Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:20383123). The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed:8621488). As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome (PubMed:32103174). Binding to U3 small nucleolar RNA, recruits PRKDC and XRCC5/Ku86 to the small-subunit processome (PubMed:32103174). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

Chromosomal Localization:

2q35

Accession Number:

NP_066964.1

UniProt Accession Number:

P13010

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

TLFPLIEAK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

990

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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