X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Peptide 2


Background

Catalog Number:

CPTC-XPNPEP1-2

Target Antigen:

X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

04/02/2015

Antigen Recognition(s):

peptide, recombinant full-length, native protein

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Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-XPNPEP-2 as primary Ab against HEK293T cell lysate containing XPNPEP (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the XPNPEP-2 Ab as the IgG control (lane 3). Click image to enlarge

CPTC-XPNPEP1-2 Western blot (cell lysate)

Western Blot using CPTC-XPNPEP-2 as primary Ab against HEK293T cell lysate containing XPNPEP (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the XPNPEP-2 Ab as the IgG control (lane 3).

  Cell Lysate Western Blot
Click to enlarge image Western Blot using CPTC-XPNPEP1-2 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-XPNPEP1-2 NCI60 Western blot

Western Blot using CPTC-XPNPEP1-2 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-XPNPEP1 (peptide 2) Peptide ELISA

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-XPNPEP1-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Background

NCI Identification Number:

00021

Antigen Name:

X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Peptide 2

CPTC Name:

CPTC-XPNPEP1 Peptide 2

Keywords:

X-Prolyl Aminopeptidase 1, Soluble; XPNPEPL; XPNPEPL1; XPNPEP; Aminoacylproline Aminopeptidase; Cytosolic Aminopeptidase P; Soluble Aminopeptidase P; X-Pro Aminopeptidase 1; EC 3.4.11.9; SAMP; X-Prolyl Aminopeptidase; (Aminopeptidase P)-Like; APP1; Aminopeptidase P, Cytosolic; Xaa-Pro Aminopeptidase 1; SAmp

Function:

This gene encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. The gene product may play a role in degradation and maturation of tachykinins, neuropeptides, and peptide hormones. Alternative splicing results in multiple transcript variants.

Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the
N-termini of peptides, such as Arg-Pro-Pro

Chromosomal Localization:

10q25.3

Expression System:

NCBI Accession Number:

NP_065116.3

Swiss Prot Accession Number:

Q9NQw7

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

TLSLDEVYLIDSGAQYK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1870

Last Updated:

06/11/2013

Links

Characterization Data

SOPs

No SOPs available.

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