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Ubiquitin-like modifier activating enzyme 1

Antibody: CPTC-UBA1-1
Background

Catalog Number:

CPTC-UBA1-1

Target Antigen:

Ubiquitin-like modifier activating enzyme 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (172.4 KB)

CPTC-UBA1-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBA1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-UBA1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500. Click image to enlarge

CPTC-UBA1-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-UBA1-1 as primary Ab against UB2L6 (rAg 00057) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-UBA1-1 Western blot

Result: Positive

Western Blot using CPTC-UBA1-1 as primary Ab against UB2L6 (rAg 00057) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-UBA1-2
Background

Catalog Number:

CPTC-UBA1-2

RRID:

AB_2617365

Target Antigen:

Ubiquitin-like modifier activating enzyme 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-UBA1-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBA1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-UBA1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-UBA1-2 as primary Ab against UB2L6 (rAg 00057) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-UBA1-2 Western blot

Result: Positive

Western Blot using CPTC-UBA1-2 as primary Ab against UB2L6 (rAg 00057) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-UBA1-3
Background

Catalog Number:

CPTC-UBA1-3

RRID:

AB_2617366

Target Antigen:

Ubiquitin-like modifier activating enzyme 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-UBA1-3 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBA1-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-UBA1-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-UBA1-3 as primary Ab against UB2L6 (rAg 00057) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-UBA1-3 Western blot

Result: Positive

Western Blot using CPTC-UBA1-3 as primary Ab against UB2L6 (rAg 00057) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Ubiquitin-like modifier activating enzyme 1
Background

NCI Identification Number:

00057

Antigen Name:

Ubiquitin-like modifier activating enzyme 1

CPTC Name:

CPTC-UBA1

Aliases:

UBE1; A1S9T; GXP1; POC20; UBE1X; Ubiquitin-Activating Enzyme E1; A1ST; AMCX1; SMAX2; A1S9; UBA1A; A1S9T And BN75 Temperature Sensitivity Complementing; POC20 Centriolar Protein Homolog; UBA1, Ubiquitin-Activating Enzyme E1 Homolog A; Ubiquitin-Like Modifier-Activating Enzyme 1; Protein A1S9

Function:

The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.

Chromosomal Localization:

XP11.23

Expression System:

E. coli

Accession Number:

NP_695012.1

UniProt Accession Number:

P22314

DNA Source:

mammalian Gene Collection

Immunogen:

Recombinant Full Length Protein

Vector Name:

pET 28-MHL

Extinction Coefficient:

Buffers:

PBS pH 7.4

Expressed Sequence:

mhhhhhhssgrenlyfqgGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVL
VSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKN
RAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVG
EFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTK
DNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFS
ICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRP
AQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQN
NLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYF
DALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGA
GAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTK
LKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALD
NVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPE
KSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERT
LRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLL
HNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTY
GLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRL
EELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIP
SADRHKSKLIAG

Native Sequence:

Calculated Isoelectric Point:

5.2

Molecular Weight:

95916

Last Updated:

10/23/2013

Links

Characterization Data

Gel

Click to enlarge image PAGE of UBA1 (rAg 00057) with molecular weight standards in lane 1
Click image to enlarge

CPTC-UBA1 PAGE

PAGE of UBA1 (rAg 00057) with molecular weight standards in lane 1

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SOPs

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