Tumor Protein P53 Binding Protein 1 Peptide 1


Background

Catalog Number:

CPTC-TP53BP1-1

Target Antigen:

Tumor Protein P53 Binding Protein 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

07/30/2021

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (341.2 KB)

CPTC-TP53BP1-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  IHC NCI60
Download Immuno-histochemistry of CPTC-TP53BP1-1 for NCI60 Cell Line Array with titer 1:1250 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) (124.9 KB)

CPTC-TP53BP1-1 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-TP53BP1-1 for NCI60 Cell Line Array with titer 1:1250
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-TP53BP1-1. Titer: 1:1250 Click image to enlarge

CPTC-TP53BP1-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-TP53BP1-1. Titer: 1:1250


  Immunofluorescence
Click to enlarge image Immunofluorescence staining of human cell line HeLa with CPTC-TP53BP1-1 Ab shows localization to the nucleus, other. Click image to enlarge

CPTC-TP53BP1-1

Result: Positive

Immunofluorescence staining of human cell line HeLa with CPTC-TP53BP1-1 Ab shows localization to the nucleus, other.


  Western Blot - Cell Lysate
Click to enlarge image Western Blot using CPTC-TP53BP1-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1). Click image to enlarge

CPTC-TP53BP1-1 western blot (cell lysates)

Result: Negative

Western Blot using CPTC-TP53BP1-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-TP53BP1-1 as primary antibody against recombinant human tumor protein p53 binding protein 1 (TP53BP1), transcript variant 3
protein (lane 2). Also included are molecular weight standards (lane 1). Click image to enlarge

CPTC-TP53BP1-1 western blot (recombinant protein)

Result: Positive

Western Blot using CPTC-TP53BP1-1 as primary antibody against recombinant human tumor protein p53 binding protein 1 (TP53BP1), transcript variant 3
protein (lane 2). Also included are molecular weight standards (lane 1).


Characterization SOP Files

Background

NCI Identification Number:

00321

Antigen Name:

Tumor Protein P53 Binding Protein 1 Peptide 1

CPTC Name:

CPTC-TP53BP1 Peptide 1

Keywords:

Tumor Protein P53 Binding Protein 1; TP53-Binding Protein 1; P53-Binding Protein 1; P53BP1; 53BP1; Tumor Suppressor P53-Binding Protein 1; Tumor Protein P53-Binding Protein, 1; Tumor Protein 53-Binding Protein, 1; TDRD30; P202

Function:

This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms.TP53BP1 (Tumor Protein P53 Binding Protein 1) is a Protein Coding gene. Diseases associated with TP53BP1 include Breast Cancer and Riddle Syndrome. Among its related pathways are Metabolism of proteins and DNA Double-Strand Break Repair. Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding and p53 binding.Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).

Chromosomal Localization:

15q15.3

NCBI Accession Number:

NP_001135451.1

UniProt Accession Number:

Q12888

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

IDEDGENT(ph)QIEDTEPMS(ph)PVLNSK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

2530

Last Updated:

02/19/2020

Links

Characterization Data

SOPs

No SOPs available.

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