Background
Catalog Number:
CPTC-TOP1MT-1
RRID:
AB_2617395
Target Antigen:
Topoisomerase I Mitochondrial
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-TOP1MT-1
Characterization Data [Compare Characterization Data]
IHC HPA
CPTC-TOP1MT-1 Evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-TOP1MT-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-TOP1MT-1 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-TOP1-1 Western blot
Result: Positive
Western Blot using CPTC-TOP1MT-1 as primary Ab against recombinant topoisomerase1 MT (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-TOP1MT-2
RRID:
AB_2617396
Target Antigen:
Topoisomerase I Mitochondrial
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-TOP1MT-2
Characterization Data [Compare Characterization Data]
IHC HPA
CPTC-TOP1MT-2 Evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-TOP1MT-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-TOP1MT-2 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-TOP1MT-2 Western blot
Result: Positive
Western Blot using CPTC-TOP1MT-2 as primary Ab against recombinant topoisomerase1 MT (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-TOP1MT-3
RRID:
AB_2617397
Target Antigen:
Topoisomerase I Mitochondrial
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-TOP1MT-3
Characterization Data [Compare Characterization Data]
IHC HPA
CPTC-TOP1MT-3 Evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-TOP1MT-3 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-TOP1MT-3 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-TOP1MT-3 Western blot
Result: Positive
Western Blot using CPTC-TOP1-3 as primary Ab against recombinant topoisomerase1 MT (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
00001
Antigen Name:
Topoisomerase I Mitochondrial
CPTC Name:
CPTC-TOP1MT
Aliases:
topoisomerase (DNA) I, mitochondrial; EC 5.99.1.2; mitochondrial DNA topoisomerase 1; TOP1mt
Function:
Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by
transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine
of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
Chromosomal Localization:
8q24.3
Expression System:
E. coli
Accession Number:
NM_052963
UniProt Accession Number:
Q969P6
DNA Source:
PEL:7387-X02-605
Immunogen:
Recombinant Full Length Protein
Vector Name:
pDest-605
Extinction Coefficient:
Buffers:
Expressed Sequence:
MRVVRLLRLRAALTLLGEVPRRPASRGVPGSRRTQKGSGARWEKEKHEDG
VKWRQLEHKGPYFAPPYEPLPDGVRF FYEGRPVRLSVAAEEVATFYGRM
LDHEYTTKEVFRKNFFNDWRKEMAVEEREVIKSLDKCDFTEIHRYFVDKA
AAR KVLSREEKQKLKEEAEKLQQEFGYCILDGHQEKIGNFKIEPPGLFR
GRGDHPKMGMLKRRITPEDVVINCSRDSKI PEPPAGHQWKEVRSDNTVT
WLAAWTESVQNSIKYIMLNPCSKLKGETAWQKFETARRLRGFVDEIRSQY
RADWKSR EMKTRQRAVALYFIDKLALRAGNEKEDGEAADTVGCCSLRVE
HVQLHPEADGCQHVVEFDFLGKDCIRYYNRVPVE KPVYKNLQLFMENKD
PRDDLFDRLTTTSLNKHLQELMDGLTAKVFRTYNASITLQEQLRALTRAE
DSIAAKILSYN RANRVVAILCNHQRATPSTFEKSMQNLQTKIQAKKEQV
AEARAELRRARAEHKAQGDGKSRSVLEKKRRLLEKLQE QLAQLSVQATD
KEENKQVALGTSKLNYLDPRISIAWCKRFRVPVEKIYSKTQRERFAWALA
MAGEDFEF
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
69872
Last Updated:
11/05/2010
Links
- EnsEMBL - EnsEMBL TOP1MT
- Entrez - Entrez TOP1MT
- Entrez - Entrez TOP1
- Uniprot - Uniprot Q969P6
Characterization Data
Gel
Click image to enlarge
CPTC-TOP1MT
PAGE of recombinant Topoisomerase1 MT (CPTC-TOP1MT) (with molecular weight standards in lane 1)
Characterization SOP Files
SOPs
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