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TIMP Metallopeptidase Inhibitor 1 Peptide 2

Antibody: CPTC-TIMP1-2
Background

Catalog Number:

CPTC-TIMP1-2

Target Antigen:

TIMP Metallopeptidase Inhibitor 1 Peptide 2

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide, Endogenous

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External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (399.5 KB)

CPTC-TIMP1-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM screening of CPTC-TIMP1-2 against synthetic peptide SEEFLIAGK (TIMP Metallopeptidase Inhibitor 1 Peptide 2) Data provided by the Carr Lab, Broad Institute https://www.broadinstitute.org/proteomics/protocols Click image to enlarge

CPTC-TIMP1-2 iMRM screening (synthetic peptide)

Result: Positive

iMRM screening of CPTC-TIMP1-2 against synthetic peptide SEEFLIAGK (TIMP Metallopeptidase Inhibitor 1 Peptide 2)

Data provided by the Carr Lab, Broad Institute
https://www.broadinstitute.org/proteomics/protocols


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line THP-1 shows localization to vesicles. Human assay: THP-1 fixed with PFA, dilution: 1:100 Human assay: U2OS fixed with PFA, dilution: 1:100 Click image to enlarge

CPTC-TIMP1-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line THP-1 shows localization to vesicles. Human assay: THP-1 fixed with PFA, dilution: 1:100
Human assay: U2OS fixed with PFA, dilution: 1:100


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-TIMP1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-TIMP1-2 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-TIMP1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

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Antigen: TIMP Metallopeptidase Inhibitor 1 Peptide 2
Background

NCI Identification Number:

00328

Antigen Name:

TIMP Metallopeptidase Inhibitor 1 Peptide 2

CPTC Name:

CPTC-TIMP1 Peptide 2

Aliases:

TIMP Metallopeptidase Inhibitor 1; Tissue Inhibitor Of Metalloproteinases 1; Fibroblast Collagenase Inhibitor; Metalloproteinase Inhibitor 1; Collagenase Inhibitor; TIMP-1; CLGI; TIMP; EPO; EPA; Tissue Inhibitor Of Metalloproteinase 1 (Erythroid Potentiating Activity, Collagenase Inhibitor); Epididymis Secretory Sperm Binding Protein; Erythroid Potentiating Activity; Erythroid-Potentiating Activity; TIMP1; HCI

Function:

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. TIMP1 (TIMP Metallopeptidase Inhibitor 1) is a Protein Coding gene. Diseases associated with TIMP1 include Oral Submucous Fibrosis and Lung Giant Cell Carcinoma. Among its related pathways are G-protein signaling Ras family GTPases in kinase cascades (scheme) and Degradation of the extracellular matrix. Gene Ontology (GO) annotations related to this gene include cytokine activity and protease binding. An important paralog of this gene is TIMP2.Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.

Chromosomal Localization:

Xp11.3

Accession Number:

XP_016885255.1

UniProt Accession Number:

P01033

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

SEEFLIAGK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

990

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

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