Transforming Growth Factor Beta 1 Peptide 1


Background

Catalog Number:

CPTC-TGFB1-1

Target Antigen:

Transforming Growth Factor Beta 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-TGFB1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6209)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-TGFB1-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-TGFB1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6209)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


  NCI 60 Protein Array

CPTC-TGFB1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-TGFB1-1 as primary antibody against human transforming growth factor, beta 1 (TGFB1) recombinant protein. The expected molecular weight is 28.4 kDa. Click image to enlarge

CPTC-TGFB1-1 Western Blot (Recombinant Protein)

Result: Positive

Western blot using CPTC-TGFB1-1 as primary antibody against human transforming growth factor, beta 1 (TGFB1) recombinant protein. The expected molecular weight is 28.4 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-TGFB1-1 Simple Western (cell lysates)

Result: Negative

Automated WB (Simple Western) using CPTC-TGFB1 as primary antibody against the whole lysate of PBMC, HeLa, Jurkat, A549, MCF7 and H226. Expected MW is 44.3 KDa. The antibody does not recognize the target in Jurkat, A549 and H226 lysates. The same cell lysates were probed with an anti-CytC antibody.


  Western Blot - Tissue or Cell Lysate

CPTC-TGFB1-1 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-TGFB1-1 as primary antibody against human HeLa (2), Jurkat (3), A549 (4), MCF7 (5) and H226 (6) whole cell lysates. The expected molecular weight is 44.3 kDa.


Characterization SOP Files

Background

NCI Identification Number:

00400

Antigen Name:

Transforming Growth Factor Beta 1 Peptide 1

CPTC Name:

CPTC-TGFB1 Peptide 1

Aliases:

Transforming Growth Factor Beta 1; Transforming Growth Factor Beta-1 Proprotein; Prepro-Transforming Growth Factor Beta-1; TGFbeta; TGFB; CED; Diaphyseal Dysplasia 1, Progressive; Transforming Growth Factor, Beta 1; Transforming Growth Factor Beta1; Camurati-Engelmann Disease; Latency-Associated Peptide; TGF-Beta-1; TGF-Beta1; IBDIMDE; TGFB1; DPD1; LAP

Function:

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. TGFB1 (Transforming Growth Factor Beta 1) is a Protein Coding gene. Diseases associated with TGFB1 include Camurati-Engelmann Disease and Inflammatory Bowel Disease, Immunodeficiency, And Encephalopathy. Among its related pathways are Simplified Interaction Map Between LOXL4 and Oxidative Stress Pathway and Transcriptional activity of SMAD2/SMAD3-SMAD4 heterotrimer. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and enzyme binding. An important paralog of this gene is TGFB3.Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.[Latency-associated peptide]: Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix (PubMed:28117447). Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1 (PubMed:2022183, PubMed:8617200, PubMed:8939931, PubMed:19750484, PubMed:22278742, PubMed:19651619). Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia (Probable). Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs) (PubMed:19750484, PubMed:22278742, PubMed:19651619). Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1 (PubMed:22278742, PubMed:28117447). Transforming growth factor beta-1: Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix (PubMed:29109152). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus (PubMed:2022183, PubMed:8617200, PubMed:8939931, PubMed:19750484, PubMed:22278742, PubMed:19651619). TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1 (PubMed:22278742, PubMed:28117447). Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (PubMed:20207738). While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules' (By similarity). Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts (By similarity). Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner (By similarity). At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development (By similarity). At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (By similarity). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (PubMed:25310401). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus (PubMed:25893292, PubMed:29483653, PubMed:30696809). Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (PubMed:25893292, PubMed:30696809).

Chromosomal Localization:

19q13.2

Accession Number:

NP_000651.3

UniProt Accession Number:

P01137

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

EAVPEPVLLSR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1210

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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