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Telomeric Repeat Binding Factor 2 Peptide 1

Antibody: CPTC-TERF2-1
Background

Catalog Number:

CPTC-TERF2-1

RRID:

AB_2617354

Target Antigen:

Telomeric Repeat Binding Factor 2 Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

08/23/2022

Antigen Recognition(s):

Peptide

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (327.0 KB)

CPTC-TERF2-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-TERF2-1 antibody with CPTC-TERF2 peptide 1 (phosphorylated) as the target antigen. Click image to enlarge

CPTC-TERF2-1 phospho peptide IPMS

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-TERF2-1 antibody with CPTC-TERF2 peptide 1 (phosphorylated) as the target antigen.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-TERF2-1 Indirect ELISA

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

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Antibody: CPTC-TERF2-2
Background

Catalog Number:

CPTC-TERF2-2

RRID:

AB_2889810

Target Antigen:

Telomeric Repeat Binding Factor 2 Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

08/23/2022

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (284.5 KB)

CPTC-TERF2-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-TERF2-2 Indirect ELISA

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

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Antigen: Telomeric Repeat Binding Factor 2 Peptide 1
Background

NCI Identification Number:

00090

Antigen Name:

Telomeric Repeat Binding Factor 2 Peptide 1

CPTC Name:

CPTC-TERF2 Peptide 1

Aliases:

Telomeric Repeat Binding Factor 2; TRBF2; TRF2; Telomeric DNA-Binding Protein; TTAGGG Repeat-Binding Factor 2; Telomeric Repeat Binding Protein 2

Function:

This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic.

TERF2 (telomeric repeat binding factor 2) is a protein-coding gene. GO annotations related to this gene include protein C-terminus binding and protein homodimerization activity. An important paralog of this gene is TERF1.

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Chromosomal Localization:

16q22.1

Accession Number:

NP_005643.2

UniProt Accession Number:

Q15554

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DLVLPTQALPA(pS)PALK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1760

Last Updated:

09/01/2020

Links

Characterization Data

SOPs

No SOPs available.

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