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Tumor-associated Calcium Signal Transducer 2

Antibody: CPTC-TACSTD2-1
Background

Catalog Number:

CPTC-TACSTD2-1

RRID:

AB_2617347

Target Antigen:

Tumor-associated Calcium Signal Transducer 2

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Download This table shows the data for pairing of the three CPTC-TACSTD2 antibodies. (792.7 KB)

CPTC-TACSTD2-1 Pairing Data

Result: Positive

This table shows the data for pairing of the three CPTC-TACSTD2 antibodies.


Characterization SOP Files

  IHC HPA

CPTC-TACSTD2-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-TACSTD2-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-TACSTD2-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-TACSTD2-1 as primary Ab against TDP2 (rAg 00007) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-TACSTD2-1 Western blot

Result: Positive

Western Blot using CPTC-TACSTD2-1 as primary Ab against TDP2 (rAg 00007) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-TACSTD2-2
Background

Catalog Number:

CPTC-TACSTD2-2

RRID:

AB_2617348

Target Antigen:

Tumor-associated Calcium Signal Transducer 2

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Download This table shows the data for pairing of the three CPTC-TACSTD2 antibodies. (792.7 KB)

CPTC-TACSTD2-2 pairing data

Result: Positive

This table shows the data for pairing of the three CPTC-TACSTD2 antibodies.


Characterization SOP Files

  IHC HPA

CPTC-TACSTD2-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-TACSTD2-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-TACSTD2-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-TACSTD2-2 as primary Ab against TDP2 (rAg 00007) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-TACSTD2-2 Western blot

Result: Positive

Western Blot using CPTC-TACSTD2-2 as primary Ab against TDP2 (rAg 00007) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-TACSTD2-3
Background

Catalog Number:

CPTC-TACSTD2-3

RRID:

AB_2617349

Target Antigen:

Tumor-associated Calcium Signal Transducer 2

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Download This table shows the data for pairing of the three CPTC-TACSTD2 antibodies. (792.7 KB)

CPTC-TACSTD2-3 pairing data

Result: Positive

This table shows the data for pairing of the three CPTC-TACSTD2 antibodies.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (170.1 KB)

CPTC-TACSTD2-3 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-TACSTD2-3. Titer: 1:25000 Click image to enlarge

CPTC-TACSTD2-3 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-TACSTD2-3. Titer: 1:25000


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane. Human assay: MCF7 fixed with PFA, dilution: 1:2000 Human assay: U-2 OS fixed with PFA, dilution: 1:2000 Click image to enlarge

CPTC-TACSTD2-3 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-TACSTD2-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-TACSTD2-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-TACSTD2-3 as primary Ab against TDP2 (rAg 00007) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-TACSTD2-3 Western blot

Result: Positive

Western Blot using CPTC-TACSTD2-3 as primary Ab against TDP2 (rAg 00007) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Tumor-associated Calcium Signal Transducer 2
Background

NCI Identification Number:

00007

Antigen Name:

Tumor-associated Calcium Signal Transducer 2

CPTC Name:

CPTC-TACSTD2

Aliases:

M1S1; EGP1; tumor-associated calcium signal transducer 2; GA7331; TROP2; GP50; GA733-1; cell surface glycoprotein TROP2; EGP-1; epithelial glycoprotein-1; Cell surface glycoprotein Trop-2; gastrointestinal tumor-associated antigen GA7331; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331; 40kD glycoprotein, identified by monoclonal antibody GA733

Function:

This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy. May function as a growth factor receptor.

Chromosomal Localization:

1p32

Expression System:

E. coli

Accession Number:

NM_002353

UniProt Accession Number:

P09758

DNA Source:

human ORFeome 1.1 collection, clone BC009409

Immunogen:

Recombinant Domain

Vector Name:

11725-X04-008

Extinction Coefficient:

Buffers:

Expressed Sequence:

QDNCTCPTNKMTVCSPDGPGGRCQCRALGSGMAVDCSTLTSKCLLLKARM
SAPKNARTLVRPSEHALVDNDGLYDPDCDPEGRFKARQCNQTSVCWCVNS
VGVRRTDKGDLSLRCDELVRTHHILIDLRHRPTAGAFNHSDLDAELRRLF
RERYRLHPKFVAAVHYEQPTIQIELRQNTSQKAAGDVDIGDAAYYFERDI
KGESLFQGRGGLDLRVRGEPLQVERTLIYYLDEIPPKFSMKRLTHHHHHH

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

28321

Last Updated:

11/20/2012

Links

Characterization Data

Gel

Click to enlarge image PAGE of TACSTD2 (Ag 00007) with molecular weight standards in lane 1
Click image to enlarge

CPTC-TACSTD2 PAGE

PAGE of TACSTD2 (Ag 00007) with molecular weight standards in lane 1

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SOPs

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