Secreted Phosphoprotein 1 Peptide 1


Background

Catalog Number:

CPTC-SPP1-1

RRID:

AB_2890093

Target Antigen:

Secreted Phosphoprotein 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

12/09/2021

Antigen Recognition(s):

Peptide, Endogenous

Characterization Data [Compare Characterization Data]
  NCI 60 Protein Array

CTPC-SPP1-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide

CPTC-SPP1-1 Western Blot Recombinant Protein

Result: Negative

Western blot using CPTC-SPP1-1 as primary antibody against human secreted phosphoprotein 1 (SPP1), transcript variant 1. Expected molecular weight - 33.7 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

Background

NCI Identification Number:

00404

Antigen Name:

Secreted Phosphoprotein 1 Peptide 1

CPTC Name:

CPTC-SPP1 Peptide 1

Keywords:

Secreted Phosphoprotein 1; OPN; BNSP; osteopontin; Early T-Lymphocyte Activation 1; Urinary Stone Protein; Bone Sialoprotein I; BSPI; ETA-1; nephropontin; Osteopontin/Immunoglobulin Alpha 1 Heavy Chain Constant Region Fusion Protein; Secreted Phosphoprotein 1 (Osteopontin, Bone Sialoprotein I, Early T-Lymphocyte Activation 1); SPP1/CALPHA1 Fusion; uropontin; SPP-1; Uropontin; Bone Sialoprotein 1

Function:

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. Theencoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor isfound in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokinethat upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encodingdifferent isoforms have been found for this gene.SPP1 (secreted phosphoprotein 1) is a protein-coding gene. Diseases associated with SPP1 include papillary cystadenocarcinoma, and calcific tendinitis. GO annotations related to this gene include extracellular matrix binding and cytokine activity.Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probablyimportant to cell-matrix interactionActs as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducingproduction of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity)

Chromosomal Localization:

4q22.1

Accession Number:

NP_001035147.1

UniProt Accession Number:

P10451

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GDSVVYGLR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

990

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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