Ribonucleotide Reductase M2 Peptide 1


Background

Catalog Number:

CPTC-RRM2-1

Target Antigen:

Ribonucleotide Reductase M2 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

11/25/2019

Antigen Recognition(s):

Peptide, Recombinant Full-length, Native Protein

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

SOP:

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Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RRM2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3255) Click image to enlarge

CPTC-RRM2-1 IMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-RRM2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3255)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-RRM2-1 peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-RRM2-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


  Western Blot - Cell Lysate
Click to enlarge image Western Blot using CPTC-RRM2-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-RRM2-1 NCI60 Western blot

Result: Positive

Western Blot using CPTC-RRM2-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).


  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-RRM2-1 as primary Ab against HEK293T cell lysate containing RRM2 (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the RRM2-1 Ab as the IgG control (lane 3). Click image to enlarge

CPTC-RRM2-1 Western blot (cell lysate)

Result: Positive

Western Blot using CPTC-RRM2-1 as primary Ab against HEK293T cell lysate containing RRM2 (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the RRM2-1 Ab as the IgG control (lane 3).


Background

NCI Identification Number:

00038

Antigen Name:

Ribonucleotide Reductase M2 Peptide 1

CPTC Name:

CPTC-RRM2 Peptide 1

Keywords:

Ribonucleotide Reductase M2 Polypeptide; Ribonucleotide Reductase Small Chain; Ribonucleotide Reductase Small Subunit; RR2; EC 1.17.4.1; R2; RR2M; Ribonucleoside-Diphosphate Reductase Subunit M2

Function:

This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the
formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a
cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1
and X.

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.

Chromosomal Localization:

2p24-p24

NCBI Accession Number:

NP_001025.1

UniProt Accession Number:

P31350

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

IEQEFLTEALPVK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1430

Last Updated:

06/21/2013

Links

Characterization Data

SOPs

No SOPs available.

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