Catalog Number:
CPTC-RNF168-1
Target Antigen:
Ring Finger Protein 168 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
07/19/2024
Antigen Recognition(s):
Peptide
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
NCI Identification Number:
00424
Antigen Name:
Ring Finger Protein 168 Peptide 1
CPTC Name:
CPTC-RNF168 Peptide 1
Aliases:
Ring Finger Protein 168; Ring Finger Protein 168, E3 Ubiquitin Protein Ligase; RING-Type E3 Ubiquitin Transferase RNF168; E3 Ubiquitin-Protein Ligase RNF168; HRNF168; RING Finger Protein 168; EC 2.3.2.27; FLJ35794; EC 6.3.2; RNF168; RIDL
Function:
This gene encodes an E3 ubiquitin ligase protein that contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The protein is involved in DNA double-strand break (DSB) repair. Mutations in this gene result in Riddle syndrome.RNF168 (Ring Finger Protein 168) is a Protein Coding gene. Diseases associated with RNF168 include Riddle Syndrome and Chromosome 3Q29 Deletion Syndrome. Among its related pathways are DNA Double Strand Break Response and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include chromatin binding and ubiquitin-protein transferase activity. An important paralog of this gene is RNF169.E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).
Chromosomal Localization:
3q29
Accession Number:
NP_689830.2
UniProt Accession Number:
Q8IYW5
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
LIDLEHLLFER
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1210
Last Updated:
09/20/2020
No SOPs available.
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