Background
Catalog Number:
CPTC-RAF1-5
RRID:
AB_2868553
Target Antigen:
Raf-1 Proto-Oncogene, Serine/Threonine Kinase Peptide 2
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
09/11/2024
Antigen Recognition(s):
Peptide, Endogenous
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-RAF1-5
External Links
- Human Protein Atlas CPTC-RAF1-5
Publications
- A dataset describing a suite of novel antibody reagents for the RAS signaling network (PMID: 31467290)
- Targeted mass-spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP kinase, and AKT signaling (PMID: 34671754)
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (512.9 KB)
CPTC-RAF1-5 evaluation by the Human Protein Atlas
Result: Positive
This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immuno-MRM
CPTC-RAF1-5 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-RAF1-5 antibody with CPTC-RAF1 peptide 2 (NCI ID#183) as target
Immunofluorescence - HPA
CPTC-RAF1-5 evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane & cytosol.
Human assay: THP-1 fixed with PFA, dilution: 1:125
Human assay: U2OS fixed with PFA, dilution: 1:125
Characterization SOP Files
Western Blot - HPA - tissue or cell lysate
CPTC-RAF1-5 evaluation by the Human Protein Atlas
Result: Positive
Single band corresponding to the predicted size in kDa (+/-20%).
Analysis performed using a standard panel of samples. Antibody dilution: 1:500
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-RAF1-5 Western Blot - Cell Lysate
Result: Negative
Western Blot using CPTC-RAF1-5 as primary antibody against the cell lysates of CCRF-CEM, T-47D, MOLT-4, SN12C, HT-29 and A498. The antibody does not recognize the target protein in the tested lysates.
Characterization SOP Files
Background
NCI Identification Number:
00183
Antigen Name:
Raf-1 Proto-Oncogene, Serine/Threonine Kinase Peptide 2
CPTC Name:
CPTC-RAF1 Peptide 2
Aliases:
Raf-1 Proto-Oncogene, Serine/Threonine Kinase; V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1; C-Raf Proto-Oncogene, Serine/Threonine Kinase; Proto-Oncogene C-RAF; EC 2.7.11.1; Raf-1; CRAF; V-Raf-1 Murine Leukemia Viral Oncogene-Like Protein 1; RAF Proto-Oncogene Serine/Threonine-Protein Kinase; Raf Proto-Oncogene Serine/Threonine Protein Kinase; Oncogene RAF1; EC 2.7.11; CMD1NN; C-Raf; NS5; RAF
Function:
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at Ser-75. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Raf kinases, a family of three serine/threonine kinases, are part of the Ras-MAPK signaling cascade and phosphorylate MEK. Upon growth factor stimulation, Raf-1 (c-Raf) is activated by GTP-bound Ras and recruited to the cell membrane. This activation process is tightly regulated.
Chromosomal Localization:
3p25
Accession Number:
NP_002871.1
UniProt Accession Number:
P04049
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
GLQPECCAVFR
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1210
Last Updated:
12/22/2016
Links
- EnsEMBL - EnsEMBL RAF
- Entrez - Entrez RAF
- Uniprot - Uniprot P04049
Characterization Data
SOPs
No SOPs available.
Don't have Adobe Reader™?
Get it for free at Adobe.com