RAD50 Homolog Peptide 1


Background

Catalog Number:

CPTC-RAD50-1

Target Antigen:

RAD50 Homolog Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

04/26/2018

Antigen Recognition(s):

peptide, phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RAD50-1 antibody (see CPTAC assay portal for details:  https://assays.cancer.gov/CPTAC-3286) Click image to enlarge

CPTC-RAD50-1 iMRM

Immuno-MRM chromatogram of CPTC-RAD50-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3286)

Background

NCI Identification Number:

00098

Antigen Name:

RAD50 Homolog Peptide 1

CPTC Name:

CPTC-RAD50 Peptide 1

Keywords:

RAD50 Homolog; NBSLD; RAD50; RAD502; DNA Repair Protein RAD50; hRAD50; EC 3.6; EC 3.6.1.15; EC 3.6.3; EC 3.6.3.27

Function:

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation,telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

RAD50 (RAD50 homolog (S. cerevisiae)) is a protein-coding gene. Diseases associated with RAD50 include nijmegen breakage syndrome-like disorder, and nijmegen breakage syndrome. GO annotations related to this gene include 3'-5' exonuclease activity and protein binding, bridging.

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

Chromosomal Localization:

5q31

Expression System:

NCBI Accession Number:

NP_005723.2

Swiss Prot Accession Number:

Q92878

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LFDVC*G(pS)QDFESDLDR (pS635)

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1760

Last Updated:

03/08/2018

Links

Characterization Data

SOPs

No SOPs available.

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