Background
Catalog Number:
CPTC-RAD50-1
RRID:
AB_2888972
Target Antigen:
RAD50 Double Strand Break Repair Protein Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
02/02/2024
Antigen Recognition(s):
Peptide, Endogenous, Phosphorylation
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
External Links
- CPTAC Assay Portal CPTAC-3286
Purchase
- DSHB CPTC-RAD50-1
External Links
- Human Protein Atlas CPTC-RAD50-1
Publications
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (494.7 KB)
CPTC-RAD50-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immuno-MRM
CPTC-RAD50-1 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-RAD50-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3286)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)
Characterization SOP Files
Immunofluorescence - HPA
CPTC-RAD50-1 evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org).
The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line U2OS shows localization to nuclear bodies.
Human assay: THP-1 fixed with PFA, dilution: 1:200
Human assay: U2OS fixed with PFA, dilution: 1:200
Characterization SOP Files
Western Blot - HPA - tissue or cell lysate
CPTC-RAD50-1 evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Single band corresponding to the predicted size in kDa (+/-20%).
Analysis performed using a standard panel of samples. Antibody dilution: 1:500
Characterization SOP Files
Background
NCI Identification Number:
00098
Antigen Name:
RAD50 Double Strand Break Repair Protein Peptide 1
CPTC Name:
CPTC-RAD50 Peptide 1
Aliases:
RAD50 Double Strand Break Repair Protein; RAD50 Homolog, Double Strand Break Repair Protein; DNA Repair Protein RAD50; RAD50 (S. Cerevisiae) Homolog; RAD50 Homolog (S. Cerevisiae); RAD502; HRad50; HRAD50; NBSLD; RAD50-2; RAD50 Protein; HRAD50
Function:
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.RAD50 (RAD50 Double Strand Break Repair Protein) is a Protein Coding gene. Diseases associated with RAD50 include Nijmegen Breakage Syndrome-Like Disorder and Inherited Cancer-Predisposing Syndrome. Among its related pathways are Sensing of DNA Double Strand Breaks and DDX1 as a regulatory component of the Drosha microprocessor.
Chromosomal Localization:
5q31.1
Accession Number:
UniProt Accession Number:
Q92878
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
LFDVC*G(pS)QDFESDLDR (pS635)
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1760
Last Updated:
07/01/2026
Links
- EnsEMBL - EnsEMBL RAD50
- Entrez - Entrez RAD50
- Uniprot - Uniprot Q92878
Characterization Data
SOPs
No SOPs available.
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