Catalog Number:
CPTC-RAD18-3
RRID:
AB_2820273
Target Antigen:
RAD18 Homolog Peptide 2
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
10/04/2024
Antigen Recognition(s):
Peptide
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immuno-MRM chromatogram of CPTC-RAD18-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3247)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Negative
Western Blot using CPTC-RAD18-3 as primary antibody against the cell lysates of SF-268, MDA-MB-231, K-562, HCC-2998, A498, and TK-10. The antibody does not recognize the target protein in the tested lysates.
Catalog Number:
CPTC-RAD18-5
RRID:
AB_2820274
Target Antigen:
RAD18 Homolog Peptide 2
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
09/11/2024
Antigen Recognition(s):
Peptide, Phosphorylation
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immuno-MRM chromatogram of CPTC-RAD18-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3247)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
Western Blot using CPTC-RAD18-5 as primary antibody against the cell lysates of SF-268, MDA-MB-231, K-562, HCC-2998, A498, and TK-10. The antibody does not recognize the target protein in the tested lysates.
NCI Identification Number:
00199
Antigen Name:
RAD18 Homolog Peptide 2
CPTC Name:
CPTC-RAD18 Peptide 2
Aliases:
RAD18 Homolog (S. Cerevisiae); Postreplication Repair Protein RAD18; RNF73; hHR18; hRAD18; RING Finger Protein 73; E3 Ubiquitin-Protein Ligase RAD18; Postreplication Repair Protein HRAD18p; RAD18, S. Cerevisiae, Homolog; EC 6.3.2.
Function:
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
RAD18 (RAD18 homolog (S. cerevisiae)) is a protein-coding gene. GO annotations related to this gene include damaged DNA binding and ubiquitin protein ligase binding.
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity
Chromosomal Localization:
3p25-p24
Accession Number:
NP_064550.3
UniProt Accession Number:
Q9NS91
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
NDLQDTEI(pS)PR (pS471)
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1210
Last Updated:
02/16/2017
No SOPs available.
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