RAD18 Homolog Peptide 2


Background

Catalog Number:

CPTC-RAD18-3

RRID:

AB_2820273

Target Antigen:

RAD18 Homolog Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

10/04/2024

Antigen Recognition(s):

Peptide

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

External Links
Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (528.5 KB)

CPTC-RAD18-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RAD18-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3247) 

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-RAD18-3 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-RAD18-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3247)

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (528.5 KB)

CPTC-RAD18-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  NCI 60 Protein Array

CPTC-RAD18-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Tissue or Cell Lysate

CPTC-RAD18-3 Western Blot - Cell Lysate

Result: Negative

Western Blot using CPTC-RAD18-3 as primary antibody against the cell lysates of SF-268, MDA-MB-231, K-562, HCC-2998, A498, and TK-10. The antibody does not recognize the target protein in the tested lysates.


Characterization SOP Files

Background

Catalog Number:

CPTC-RAD18-5

RRID:

AB_2820274

Target Antigen:

RAD18 Homolog Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

09/11/2024

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

External Links
Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (561.8 KB)

CPTC-RAD18-5 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RAD18-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3247) 

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-RAD18-5 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-RAD18-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3247)

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (561.8 KB)

CPTC-RAD18-5 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Western Blot - Tissue or Cell Lysate

CPTC-RAD18-5 Western Blot - Cell Lysate

Result: Negative

Western Blot using CPTC-RAD18-5 as primary antibody against the cell lysates of SF-268, MDA-MB-231, K-562, HCC-2998, A498, and TK-10. The antibody does not recognize the target protein in the tested lysates.


Characterization SOP Files

Background

NCI Identification Number:

00199

Antigen Name:

RAD18 Homolog Peptide 2

CPTC Name:

CPTC-RAD18 Peptide 2

Aliases:

RAD18 Homolog (S. Cerevisiae); Postreplication Repair Protein RAD18; RNF73; hHR18; hRAD18; RING Finger Protein 73; E3 Ubiquitin-Protein Ligase RAD18; Postreplication Repair Protein HRAD18p; RAD18, S. Cerevisiae, Homolog; EC 6.3.2.

Function:

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

RAD18 (RAD18 homolog (S. cerevisiae)) is a protein-coding gene. GO annotations related to this gene include damaged DNA binding and ubiquitin protein ligase binding.

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity

Chromosomal Localization:

3p25-p24

Accession Number:

NP_064550.3

UniProt Accession Number:

Q9NS91

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

NDLQDTEI(pS)PR (pS471)

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1210

Last Updated:

02/16/2017

Links

Characterization Data

SOPs

No SOPs available.

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