PRINT

Phosphoserine phosphatase-like

Antibody: CPTC-PSPHL-1
Background

Catalog Number:

CPTC-PSPHL-1

RRID:

AB_2617318

Target Antigen:

Phosphoserine phosphatase-like

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

11/25/2019

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

FSAI003R-3G9 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-PSPHL-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-PSPHL-1 Protein Array

Result: Positive

Protein Array in which CPTC-PSPHL-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PSPHL-1 as primary Ab against PSPHL (Ag 00002) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

FSAI003R-3G9 Western blot

Result: Positive

Western Blot using CPTC-PSPHL-1 as primary Ab against PSPHL (Ag 00002) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

[top]

Antibody: CPTC-PSPHL-2
Background

Catalog Number:

CPTC-PSPHL-2

RRID:

AB_10805280

Target Antigen:

Phosphoserine phosphatase-like

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

11/25/2019

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

FSAI003R-3E10 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-PSPHL-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-PSPHL-2 Protein Array

Result: Positive

Protein Array in which CPTC-PSPHL-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PSPHL-2 as primary Ab against PSPHL (Ag 00002) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

FSAI003R-3E10 Western blot

Result: Positive

Western Blot using CPTC-PSPHL-2 as primary Ab against PSPHL (Ag 00002) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

[top]

Antibody: CPTC-PSPHL-3
Background

Catalog Number:

CPTC-PSPHL-3

RRID:

AB_2617319

Target Antigen:

Phosphoserine phosphatase-like

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

11/25/2019

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC NCI60

CPTC-PSPHL-3 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-PSPHL-3 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

FSAI003R-2F10 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PSPHL-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PSPHL-3 as primary Ab against PSPHL (Ag 00002) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

FSAI003R-2F10 Western blot

Result: Positive

Western Blot using CPTC-PSPHL-3 as primary Ab against PSPHL (Ag 00002) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

[top]

Antigen: Phosphoserine phosphatase-like
Background

NCI Identification Number:

00002

Antigen Name:

Phosphoserine phosphatase-like

CPTC Name:

CPTC-PSPHL

Aliases:

phosphoserine phosphatase-like; CO9; L-3-phosphoserine-phosphatase homolog

Function:

This gene is significantly upregulated in Fanconi's anemia fibroblasts but downregulated or absent in fibroblasts
from normal donors. It is also highly expressed in FA B-cells of complementation group A.

Chromosomal Localization:

7p11.2

Expression System:

E. coli

Accession Number:

XM_926720

UniProt Accession Number:

O15172

DNA Source:

xxx

Immunogen:

Recombinant Full Length Protein

Vector Name:

pDest 527

Extinction Coefficient:

Buffers:

Expressed Sequence:

MRSGSHHHHHHRSDITSLYKKVGENLYFQGASASCSPGGALASPEPGRKI
LPRMISHSELRKLFYSADAVCF

Native Sequence:

Calculated Isoelectric Point:

7.8

Molecular Weight:

11200

Last Updated:

12/16/2010

Links

Characterization Data

Gel

Click to enlarge image PAGE of Ag 00002 (PSPHL) (with molecular weight standards in lane 1)
Click image to enlarge

CPTC-PSPHL PAGE

PAGE of Ag 00002 (PSPHL) (with molecular weight standards in lane 1)

No SOPs available.

[top]

SOPs

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]