Catalog Number:
CPTC-PP2A-1
RRID:
AB_1553763
Target Antigen:
Protein Phosphatase 2A
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
05/28/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
Kinetic titration data for PP2A-1 Ab (622A1.1) using Biacore SPR method
Result: Positive
Imaging mass cytometry on lung cancer tissue core using CPTC-PP2A-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (breast and endometrium) and cancer tissues (breast, colon, ovarian, lung, and prostate).
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500
Result: Positive
Western Blot using CPTC-PP2A-1 as primary Ab against Ag 10501 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-PP2A-2
RRID:
AB_1553765
Target Antigen:
Protein Phosphatase 2A
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/01/2022
Antigen Recognition(s):
Recombinant Full-length
SOP:
Result: Positive
Kinetic titration data for PP2A-2 Ab (622A3.1) using Biacore SPR method
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Western Blot using CPTC-PP2A-2 as primary Ab against Ag 10501 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-PP2A-3
RRID:
AB_10571459
Target Antigen:
Protein Phosphatase 2A
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
05/28/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
Imaging mass cytometry on normal colon tissue core using CPTC-PP2A-3 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, placenta, prostate, colon, pancreas, breast, lung, endometrium, appendix, and kidney) and cancer tissues (breast, colon, ovarian, lung, and prostate).
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-PP2A-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Western Blot using CPTC-PP2A-3 as primary Ab against Ag 10501 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-PP2A-4
RRID:
AB_2169787
Target Antigen:
Protein Phosphatase 2A
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
05/28/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
Imaging mass cytometry on normal prostate tissue core using CPTC-PP2A-4 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, spleen, prostate, colon, pancreas, breast, testis, endometrium, appendix, and kidney) and cancer tissues (breast, colon, ovarian, lung, and prostate).
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Western Blot using CPTC-PP2A-4 as primary Ab against Ag 10501 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
NCI Identification Number:
10501
Antigen Name:
Protein Phosphatase 2A
CPTC Name:
CPTC-PP2A
Aliases:
PP2A; PPP2CA; EC3.1.3.16; PP2A-alpha; PP2Ac; PP2CA; RP-C
Function:
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53.
Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than
the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen
Chromosomal Localization:
5q31.1
Expression System:
E. Coli
Accession Number:
BC011605
UniProt Accession Number:
Q15257
DNA Source:
HIP:HsCD00004559
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
56505
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMAEGERQPPPDSSEEAPPATQNFIIPKKEIHTVPDMGKWKRSQAYAD
YIGFILTLNEGVKGKKLTFEYRVSEAIEKLVALLNTLDRWIDETPPVDQP
SRFGNKAYRTWYAKLDEEAENLVATVVPTHLAAAVPEVAVYLKESVGNST
RIDYGTGHEAAFAAFLCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQK
TYRMEPAGSQGVWGLDDFQFLPFIWGSSQLIDHPYLEPRHFVDEKAVNEN
HKDYMFLECILFITEMKTGPFAEHSNQLWNISAVPSWSKVNQGLIRMYKA
ECLEKFPVIQHFKFGSLLPIHPVTSG
Native Sequence:
MAEGERQPPPDSSEEAPPATQNFIIPKKEIHTVPDMGKWKRSQAYADYIG
FILTLNEGVKGKKLTFEYRVSEAIEKLVALLNTLDRWIDETPPVDQPSRF
GNKAYRTWYAKLDEEAENLVATVVPTHLAAAVPEVAVYLKESVGNSTRID
YGTGHEAAFAAFLCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYR
MEPAGSQGVWGLDDFQFLPFIWGSSQLIDHPYLEPRHFVDEKAVNENHKD
YMFLECILFITEMKTGPFAEHSNQLWNISAVPSWSKVNQGLIRMYKAECL
EKFPVIQHFKFGSLLPIHPVTSG
Calculated Isoelectric Point:
5.89
Molecular Weight:
37047
Last Updated:
09/01/2008
PAGE of Ag 10501 with molecular weight standards in lane 1
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