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Phosphatidylethanolamine Binding Protein 1

Antibody: CPTC-PEBP1-1
Background

Catalog Number:

CPTC-PEBP1-1

Target Antigen:

Phosphatidylethanolamine Binding Protein 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

11/25/2019

Antigen Recognition(s):

Recombinant Full-length

Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PEBP1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-PEBP1-1 Western blot

Result: Negative

This antibody is not suitable for use in Western blots


Characterization SOP Files

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Antibody: CPTC-PEBP1-2
Background

Catalog Number:

CPTC-PEBP1-2

RRID:

AB_2617308

Target Antigen:

Phosphatidylethanolamine Binding Protein 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Recombinant Full-length

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (592.8 KB)

CPTC-PEBP1-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (592.8 KB)

CPTC-PEBP1-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PEBP1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PEBP1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PEBP1-2 as primary Ab against PEBP1 (rAg 00060) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-PEBP1-2 Western blot

Result: Positive

Western Blot using CPTC-PEBP1-2 as primary Ab against PEBP1 (rAg 00060) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Phosphatidylethanolamine Binding Protein 1
Background

NCI Identification Number:

00060

Antigen Name:

Phosphatidylethanolamine Binding Protein 1

CPTC Name:

CPTC-PEBP1

Aliases:

PBP; RKIP; PEBP; HCNP; Prostatic Binding Protein; Neuropolypeptide H3; Prostatic-Binding Protein; Raf Kinase Inhibitor Protein; HCNPpp; PEBP-1; Hippocampal Cholinergic Neurostimulating Peptide; Phosphatidylethanolamine-Binding Protein 1; Raf Kinase Inhibitory Protein

Function:

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation

Function: HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.

Chromosomal Localization:

12q24.23

Expression System:

Baculovirus

Accession Number:

NP_002558.1

UniProt Accession Number:

P30086

DNA Source:

human ORFeome library

Immunogen:

Recombinant Full Length Protein

Vector Name:

11725-X06-635

Extinction Coefficient:

Buffers:

1x PBS, pH 7.2

Expressed Sequence:

MGSGSHHHHHHRSSITSLYKKVGENLYFQGPVDLSKWSGPLSLQEVDEQP
QHPLHVTYAGAAVDELGKVLTPTQVKNRPTSISWDGLDSGKLYTLVLTDP
DAPSRKDPKYREWHHFLVVNMKGNDISSGTVLSDYVGSGPPKGTGLHRYV
WLVYEQDRPLKCDEPILSNRSGDHRGKFKVASFRKKYELRAPVAGTCYQA
EWDDYVPKLYEQLSGKHHHHHH

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

25163

Last Updated:

08/21/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of PEBP1 (rAg 00060) with molecular weight standards in lane 1
Click image to enlarge

CPTC-PEBP1 PAGE

PAGE of PEBP1 (rAg 00060) with molecular weight standards in lane 1

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SOPs

No SOPs available.

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