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Proliferating Cell Nuclear Antigen Peptide 2

Antibody: CPTC-PCNA-4
Background

Catalog Number:

CPTC-PCNA-4

RRID:

AB_2888966

Target Antigen:

Proliferating Cell Nuclear Antigen Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

08/03/2026

Antigen Recognition(s):

Peptide, Endogenous, Ubiquitination

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

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Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on ovarian cancer tissue core using CPTC-PCNA-4 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, prostate, liver, colon, endometrium, appendix, pancreas, bone marrow, breast, kidney, lung, and placenta) and cancer tissues (breast, lung, prostate, colon and ovarian). Click image to enlarge

CPTC-PCNA-4 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on ovarian cancer tissue core using CPTC-PCNA-4 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, prostate, liver, colon, endometrium, appendix, pancreas, bone marrow, breast, kidney, lung, and placenta) and cancer tissues (breast, lung, prostate, colon and ovarian).


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (137.9 KB)

CPTC-PCNA-4 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

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Antibody: CPTC-PCNA-5
Background

Catalog Number:

CPTC-PCNA-5

RRID:

AB_2877661

Target Antigen:

Proliferating Cell Nuclear Antigen Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

08/03/2026

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Ubiquitination

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

Purchase
External Links
Publications
Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on breast cancer tissue core using CPTC-PCNA-5 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, prostate, liver, colon, endometrium, appendix, pancreas, bone marrow, breast, kidney, lung, and placenta) and cancer tissues (colon, prostate, lung, and ovarian). Click image to enlarge

CPTC- PCNA-5 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on breast cancer tissue core using CPTC-PCNA-5 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, prostate, liver, colon, endometrium, appendix, pancreas, bone marrow, breast, kidney, lung, and placenta) and cancer tissues (colon, prostate, lung, and ovarian).


Characterization SOP Files

  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (158.3 KB)

CPTC-PCNA-5 Evaluation by the Human Protein Atlas

Result: Positive

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM analysis using CPTC-PCNA-5 as capture antibody. Antibody CPTC-PCNA-5 is able to recognize the tryptic peptide of PCNA in its ubiquitinated form [Panel A, DLSHIGDAVVISCA(K-ε-GG)DGVK (uK164)], and in its not ubiquitinated form [Panel B, DLSHIGDAVVISCAKDGVK). Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-PCNA-5 iMRM (synthetic peptide)

Result: Positive

iMRM analysis using CPTC-PCNA-5 as capture antibody. Antibody CPTC-PCNA-5 is able to recognize the tryptic peptide of PCNA in its ubiquitinated form [Panel A, DLSHIGDAVVISCA(K-ε-GG)DGVK (uK164)], and in its not ubiquitinated form [Panel B, DLSHIGDAVVISCAKDGVK).
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PNCA-5 as primary Ab against recombinant PNCA protein (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-PCNA-5 WB against Recombinant Protein

Result: Positive

Western Blot using CPTC-PNCA-5 as primary Ab against recombinant PNCA protein (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: Proliferating Cell Nuclear Antigen Peptide 2
Background

NCI Identification Number:

00095

Antigen Name:

Proliferating Cell Nuclear Antigen Peptide 2

CPTC Name:

CPTC-PCNA Peptide 2

Aliases:

DNA Polymerase Delta Auxiliary Protein; cyclin; Proliferating Cell Nuclear Antigen

Function:

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Chromosomal Localization:

20 pter-P12

Accession Number:

NP_002583.1

UniProt Accession Number:

P12004

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DLSHIGDAVVISCA(K-ε-GG)DGVK (uK164)

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2310

Last Updated:

10/01/2020

Links

Characterization Data

SOPs

No SOPs available.

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