Background
Catalog Number:
CPTC-PCNA-3
RRID:
AB_2888965
Target Antigen:
Proliferating Cell Nuclear Antigen Peptide 3
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
05/22/2023
Antigen Recognition(s):
Peptide, Endogenous
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
External Links
- CPTAC Assay Portal CPTC-PCNA-3 iMRM
Purchase
- DSHB CPTC-PCNA-3
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (164.1 KB)
CPTC-PCNA-3 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC Tissue
CPTC-PCNA-3 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Immuno-MRM
CPTC-PCNA-3 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-PCNA-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3243)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)
Characterization SOP Files
Background
NCI Identification Number:
00190
Antigen Name:
Proliferating Cell Nuclear Antigen Peptide 3
CPTC Name:
CPTC-PCNA Peptide 3
Aliases:
DNA Polymerase Delta Auxiliary Protein; cyclin; Proliferating Cell Nuclear Antigen
Function:
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Chromosomal Localization:
20 pter-P12
Accession Number:
NP_002583.1
UniProt Accession Number:
P12004
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
AEDNADTLALVFEAPNQEK
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
2090
Last Updated:
02/16/2017
Links
- EnsEMBL - EnsEMBL PCNA
- Entrez - Entrez PCNA
- Uniprot - Uniprot P12004
Characterization Data
SOPs
No SOPs available.
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