Proliferating Cell Nuclear Antigen Peptide 2


Background

Catalog Number:

CPTC-PCNA-2

RRID:

AB_2877661

Target Antigen:

Proliferating Cell Nuclear Antigen Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

10/22/2021

Antigen Recognition(s):

Peptide, Recombinant Full-length, Ubiquitination

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

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Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image mmuno-MRM chromatogram of CPTC-PCNA-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3245) Click image to enlarge

CPTC-PCNA-2 iMRM

Result: Positive

mmuno-MRM chromatogram of CPTC-PCNA-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3245)


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PNCA-2 as primary Ab against recombinant PNCA protein (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-PCNA-2 WB against Recombinant Protein

Result: Positive

Western Blot using CPTC-PNCA-2 as primary Ab against recombinant PNCA protein (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

Background

Catalog Number:

CPTC-PCNA-4

RRID:

AB_2888966

Target Antigen:

Proliferating Cell Nuclear Antigen Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

10/22/2021

Antigen Recognition(s):

Peptide, Ubiquitination

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

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Background

NCI Identification Number:

00095

Antigen Name:

Proliferating Cell Nuclear Antigen Peptide 2

CPTC Name:

CPTC-PCNA Peptide 2

Keywords:

DNA Polymerase Delta Auxiliary Protein; cyclin; Proliferating Cell Nuclear Antigen

Function:

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Chromosomal Localization:

20 pter-P12

NCBI Accession Number:

NP_002583.1

UniProt Accession Number:

P12004

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DLSHIGDAVVISCA(K-ε-GG)DGVK (uK164)

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2310

Last Updated:

10/01/2020

Links

Characterization Data

SOPs

No SOPs available.

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