Proliferating Cell Nuclear Antigen Peptide 1


Background

Catalog Number:

CPTC-PCNA-1

RRID:

AB_2617307

Target Antigen:

Proliferating Cell Nuclear Antigen Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

08/23/2022

Antigen Recognition(s):

Peptide

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (352.7 KB)

CPTC-PCNA-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PCNA-1 Indirect ELISA (peptide)

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PCNA-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Background

NCI Identification Number:

00035

Antigen Name:

Proliferating Cell Nuclear Antigen Peptide 1

CPTC Name:

CPTC-PCNA Peptide 1

Aliases:

Cyclin; DNA Polymerase Delta Auxiliary Protein

Function:

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Chromosomal Localization:

20pter-p12

Accession Number:

NP_002583.1

UniProt Accession Number:

P12004

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LVQGSILK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

880

Last Updated:

06/11/2013

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Characterization Data

SOPs

No SOPs available.

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