Poly(ADP-Ribose) Polymerase 1 Peptide 1


Background

Catalog Number:

CPTC-PARP1-1

Target Antigen:

Poly(ADP-Ribose) Polymerase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

04/26/2018

Antigen Recognition(s):

peptide, phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PARP1-1 antibody (see CPTAC assay portal for details:  https://assays.cancer.gov/CPTAC-3241) Click image to enlarge

CPTC-PARP1-1 iMRM

Immuno-MRM chromatogram of CPTC-PARP1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3241)

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PARP1-1 antibody reactivity to the non-phosphorylated peptide(see CPTAC assay portal for details:  https://assays.cancer.gov/CPTAC-3240) Click image to enlarge

CPTC-PARP1-1 iMRM (non-phosho)

Immuno-MRM chromatogram of CPTC-PARP1-1 antibody reactivity to the non-phosphorylated peptide(see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3240)

Background

NCI Identification Number:

00198

Antigen Name:

Poly(ADP-Ribose) Polymerase 1 Peptide 1

CPTC Name:

CPTC-PARP1 Peptide 1

Keywords:

Poly(ADP-Ribose) Polymerase 1; ADP-Ribosyltransferase (NAD+; Poly (ADP-Ribose) Polymerase); ADP-Ribosyltransferase Diphtheria Toxin-Like 1; Poly (ADP-Ribose) Polymerase Family, Member 1; NAD(+) ADP-Ribosyltransferase 1; Poly[ADP-Ribose] Synthase 1; EC 2.4.2.30; ADPRT 1; PARP-1; ADPRT; ARTD1; PPOL; Poly [ADP-Ribose] Polymerase 1; ADP-Ribosyltransferase NAD(+); Poly(ADP-Ribosyl)Transferase; Poly(ADP-Ribose) Polymerase; Poly(ADP-Ribose) Synthetase; PADPRT-1; EC 2.4.2; ADPRT1; PARP

Function:

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

PARP1 (Poly(ADP-Ribose) Polymerase 1) is a Protein Coding gene. Diseases associated with PARP1 include Diphtheria and Hemorrhagic Cystitis. Among its related pathways are Granzyme Pathway and Signaling by GPCR. GO annotations related to this gene include poly(A) RNA binding and protein kinase binding. An important paralog of this gene is PARP2.

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:17177976, PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed:17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed:17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed:23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). Mediates the poly(ADP-ribosyl)ation of histones in a C4orf27/HPF1-dependent manner (PubMed:27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming.

Poly (ADP-ribose) polymerase (PARP) catalyzes the post-translational modification of proteins by the addition of multiple ADP-ribose moieties. PARP transfers ADP-ribose from nicotinamide dinucleotide (NAD) to Glu/Asp residues on the substrate protein.

Chromosomal Localization:

1q41-q42

Expression System:

NCBI Accession Number:

NP_001609.2

Swiss Prot Accession Number:

P09874

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

EELGFRPEY(pS)ASQLK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1650

Last Updated:

02/16/2017

Links

Characterization Data

SOPs

No SOPs available.

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