Background
Catalog Number:
CPTC-OTUB2-1
RRID:
AB_2617305
Target Antigen:
OTU domain, ubiquitin aldehyde binding 2
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
09/01/2022
Antigen Recognition(s):
Recombinant Full-length
SOP:
Purchase
- DSHB CPTC-OTUB2-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (577.3 KB)
CPTC-OTUB2-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (577.3 KB)
CPTC-OTUB2-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-OTUB2-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-OTUB2-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-OTUB2-1 Western blot
Result: Positive
Western Blot using CPTC-OTUB2-1 as primary Ab against PEBP1 (rAg 00054) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-OTUB2-2
RRID:
AB_2617306
Target Antigen:
OTU domain, ubiquitin aldehyde binding 2
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
01/04/2023
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-OTUB2-2
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (552.6 KB)
CPTC-OTUB2-2 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (552.6 KB)
CPTC-OTUB2-2 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-OTUB2-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-OTUB2-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - HPA - tissue or cell lysate
CPTC-OTUB2-2 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-OTUB2-2 Western blot
Result: Positive
Western Blot using CPTC-OTUB2-2 as primary Ab against PEBP1 (rAg 00054) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
00054
Antigen Name:
OTU domain, ubiquitin aldehyde binding 2
CPTC Name:
CPTC-OTUB2
Aliases:
C14orf137; OTB2; OTU2; Deubiquitinating Enzyme OTUB2; Ubiquitin-Specific-Processing Protease OTUB2; OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 2; FLJ21916; MGC3102; Chromosome 14 Open Reading Frame 137; Otubain-2; Ubiquitin Thioesterase OTUB2; Ubiquitin-Specific Protease Otubain 2; EC 3.4.19.12
Function:
This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain.
Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
Chromosomal Localization:
14q32.12
Expression System:
E.coli
Accession Number:
NM_023112
UniProt Accession Number:
Q96DC9
DNA Source:
Imagenes
Immunogen:
Native Protein
Vector Name:
pNIC28-Bsa4
Extinction Coefficient:
Buffers:
NaP
Expressed Sequence:
MHHHHHHSSGVDLGTENLYFQSMETSFNLISEKCDILSILRDHPENRIYR
RKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSREIFKFKERVL
QTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDGSVSSLLKVFNDQSASD
HIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQ
ITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNILY
AADKH
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
29679
Last Updated:
06/17/2020
Links
- EnsEMBL - EnsEMBL OTUB2
- Entrez - Entrez OTUB2
- Uniprot - Uniprot Q96DC9
Characterization Data
Gel
Click image to enlarge
CPTC-OTUB2 PAGE
PAGE of OTUB2 (rAg 00054) with molecular weight standards in lane 1
Characterization SOP Files
SOPs
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