8-oxoguanine DNA glycosylase


Background

Catalog Number:

CPTC-OGG1-1

Target Antigen:

8-oxoguanine DNA glycosylase

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

02/26/2013

Antigen Recognition(s):

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-OGG1-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

  Western Blot
Click to enlarge image Western Blot using CPTC-OGG1-1 as primary Ab against Ag 10783 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-OGG1-1 Western blot

Western Blot using CPTC-OGG1-1 as primary Ab against Ag 10783 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  NCI 60 Protein Array

CPTC-OGG1-1 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (144.7 KB)

CPTC-OGG1-1 Evaluation by the Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

  IHC Tissue

CPTC-OGG1-1 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

  IHC NCI60

CPTC-OGG1-1 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Background

NCI Identification Number:

10783

Antigen Name:

8-oxoguanine DNA glycosylase

CPTC Name:

CPTC-OGG1

Keywords:

HMMH; HOGG1; MMH; MUTM; OGH1; OTTHUMP00000122619

Function:

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined.


DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.

Chromosomal Localization:

3p26.2

Expression System:

E. Coli

NCBI Accession Number:

BC000657.2

Swiss Prot Accession Number:

O15527

DNA Source:

HIP : HsCD00041846

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

68660

Buffers:

PBS

Expressed Sequence:

SNAMPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRW
REQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVR
KYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICS
SNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLR
KLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVG
TKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNK
ELGNFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG

Native Sequence:

MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQ
SPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYF
QLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNN
NIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLG
LGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKV
ADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELG
NFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG

Calculated Isoelectric Point:

8.84

Molecular Weight:

38.8

Last Updated:

11/14/2012

Links

Characterization Data

Gel

Click to enlarge image PAGE of OGG1 (Ag 10783) in Lanes 2 & 3 with molecular weight standards in lane 1
Click image to enlarge

CPTC-OGG1 PAGE

PAGE of OGG1 (Ag 10783) in Lanes 2 & 3 with molecular weight standards in lane 1

No SOPs available.

SOPs

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