8-oxoguanine DNA glycosylase


Background

Catalog Number:

CPTC-OGG1-1

Target Antigen:

8-oxoguanine DNA glycosylase

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

02/26/2013

Antigen Recognition(s):

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Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-OGG1-1 as primary Ab against Ag 10783 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-OGG1-1 Western blot

Western Blot using CPTC-OGG1-1 as primary Ab against Ag 10783 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-OGG1-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

  NCI 60 Protein Array

CPTC-OGG1-1 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

  IHC NCI60

CPTC-OGG1-1 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example
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IHC Cell Line Images

IHC NCI60 Cell Line Array example
  IHC Tissue

CPTC-OGG1-1 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

  IHC HPA
Download CPTC-OGG1-1.pdf (144.7 KB)

CPTC-OGG1-1 Evaluation by the Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Background

NCI Identification Number:

10783

Antigen Name:

8-oxoguanine DNA glycosylase

CPTC Name:

CPTC-OGG1

Keywords:

HMMH; HOGG1; MMH; MUTM; OGH1; OTTHUMP00000122619

Function:

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined.


DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.

Chromosomal Localization:

3p26.2

Expression System:

E. Coli

NCBI Accession Number:

BC000657.2

Swiss Prot Accession Number:

O15527

DNA Source:

HIP : HsCD00041846

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

68660

Buffers:

PBS

Expressed Sequence:

SNAMPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRW
REQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVR
KYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICS
SNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLR
KLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVG
TKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNK
ELGNFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG

Native Sequence:

MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQ
SPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYF
QLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNN
NIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLG
LGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKV
ADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELG
NFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG

Calculated Isoelectric Point:

8.84

Molecular Weight:

38.8

Last Updated:

11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click image to enlarge

CPTC-OGG1 PAGE

PAGE of OGG1 (Ag 10783) in Lanes 2 & 3 with molecular weight standards in lane 1

SOPs

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