Background
Catalog Number:
CPTC-OGG1-1
RRID:
AB_10805279
Target Antigen:
8-Oxoguanine DNA Glycosylase
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
05/28/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-OGG1-1
External Links
- Human Protein Atlas CPTC- OGG1-1
Characterization Data [Compare Characterization Data]
CyTOF
CPTC-OGG1-1 Immuno-Mass Cytometry
Result: Positive
Imaging mass cytometry on normal liver tissue core using CPTC-OGG1-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissue (liver) and cancer tissues (breast, colon, ovarian, lung, and prostate).
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (144.7 KB)
CPTC-OGG1-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
CPTC-OGG1-1 IHC NCI60
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-OGG1-1 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Immunofluorescence - HPA
CPTC-OGG1-1 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Human assay: A-431 fixed with PFA, dilution: 1:200
Human assay: U-2 OS fixed with PFA, dilution: 1:200
Human assay: U-251 MG fixed with PFA, dilution: 1:200
Characterization SOP Files
Indirect ELISA
CPTC-OGG1-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-OGG1-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - HPA - tissue or cell lysate
CPTC-OGG1-1 Evaluation by the Human Protein Atlas
Result: Presumed Positive (with additional bands)
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-OGG1-1 Western blot
Result: Positive
Western Blot using CPTC-OGG1-1 as primary Ab against Ag 10783 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
10783
Antigen Name:
8-Oxoguanine DNA Glycosylase
CPTC Name:
CPTC-OGG1
Aliases:
HMMH; HOGG1; MMH; MUTM; OGH1; OTTHUMP00000122619
Function:
This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined.
DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
Chromosomal Localization:
3p26.2
Expression System:
E. Coli
Accession Number:
BC000657.2
UniProt Accession Number:
O15527
DNA Source:
HIP : HsCD00041846
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
68660
Buffers:
PBS
Expressed Sequence:
SNAMPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRW
REQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVR
KYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICS
SNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLR
KLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVG
TKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNK
ELGNFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG
Native Sequence:
MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQ
SPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYF
QLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNN
NIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLG
LGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKV
ADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELG
NFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG
Calculated Isoelectric Point:
8.84
Molecular Weight:
38.8
Last Updated:
08/22/2020
Links
- EnsEMBL - EnsEMBL OGG1
- Entrez - Entrez OGG1
- Uniprot - Uniprot O15527
Characterization Data
Gel
Click image to enlarge
CPTC-OGG1 PAGE
PAGE of OGG1 (Ag 10783) in Lanes 2 & 3 with molecular weight standards in lane 1
Characterization SOP Files
SOPs
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