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5'-Nucleotidase Ecto Peptide 1

Antibody: CPTC-NT5E-1
Background

Catalog Number:

CPTC-NT5E-1

Target Antigen:

5'-Nucleotidase Ecto Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

04/19/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

External Links
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (484.7 KB)

CPTC-NT5E-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-NT5E-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5952) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-NT5E-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-NT5E-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5952)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (484.7 KB)

CPTC-NT5E-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA

CPTC-NT5E-1 Indirect ELISA

Result: Negative

Indirect ELISA using CPTC-NT5E-1 as primary antibody against full length NT5E protein. Full length protein was coated on the plate and was detected using goat anti-rabbit secondary antibody and TMB. Data was fit using a four parameter logistic (4PL) regression model.


Characterization SOP Files

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Antigen: 5'-Nucleotidase Ecto Peptide 1
Background

NCI Identification Number:

00454

Antigen Name:

5'-Nucleotidase Ecto Peptide 1

CPTC Name:

CPTC-NT5E Peptide 1

Aliases:

5'-Nucleotidase Ecto; Ecto-5'-Nucleotidase; 5'-Nucleotidase; EC 3.1.3.5; CALJA; 5'-NT; CD73; NT5; NTE; ENT; EN; 5'-Nucleotidase, Ecto (CD73); Purine 5-Prime-Nucleotidase; 5' Nucleotidase (CD73); CD73 Antigen; E5NT; NT5E; NT

Function:

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.NT5E (5'-Nucleotidase Ecto) is a Protein Coding gene. Diseases associated with NT5E include Calcification Of Joints And Arteries and Intestinal Tuberculosis. Among its related pathways are Neuroscience and ATP/ITP metabolism. Gene Ontology (GO) annotations related to this gene include nucleotide binding and hydrolase activity, acting on ester bonds.Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Chromosomal Localization:


6q14.3

Accession Number:

NP_002517.1

UniProt Accession Number:

P21589

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

VIYPAVEGR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

990

Last Updated:

10/01/2020

Links

Characterization Data

SOPs

No SOPs available.

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