Nucleoside Diphosphate Kinase B


Background

Catalog Number:

CPTC-NME2-1

Target Antigen:

Nucleoside Diphosphate Kinase B

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

10/10/2008

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Click to enlarge image Kinetic titration data for NME2-1 Ab (606A1.1) using Biacore SPR method Click image to enlarge

CPTC-NME2-1 SPR

Result: Positive

Kinetic titration data for NME2-1 Ab (606A1.1) using Biacore SPR method


  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity between NME1 and NME2. Click image to enlarge

Cross Reactivity Studies

Result: Positive

This table shows the cross reactivity between NME1 and NME2.


Characterization SOP Files

  IPMS
Download This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry. (123.0 KB)

CPTC-NME2-1 Immuno-MS

Result: Positive

This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.


  Indirect ELISA
Click to enlarge image Indirect ELISA of CPTC-NME2-1 Click image to enlarge

CPTC-NME2-1 ELISA

Result: Positive

Indirect ELISA of CPTC-NME2-1


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot Using CPTC-NME2-1 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3). Click image to enlarge

CPTC-NME2-1 Western Blot

Result: Positive

Western Blot Using CPTC-NME2-1 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).


Background

Catalog Number:

CPTC-NME2-2

Target Antigen:

Nucleoside Diphosphate Kinase B

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

11/25/2019

Antigen Recognition(s):

Recombinant Full-length, Native Protein

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Click to enlarge image Kinetic titration data for NME2-2 Ab (606A13.1) using Biacore SPR method Click image to enlarge

CPTC-NME2-2 SPR

Result: Positive

Kinetic titration data for NME2-2 Ab (606A13.1) using Biacore SPR method


  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity between NME1 and NME2. Click image to enlarge

Cross Reactivity Studies

Result: Positive

This table shows the cross reactivity between NME1 and NME2.


Characterization SOP Files

  IHC NCI60
Click to enlarge image Immunohistochemistry of CPTC-NME2-2 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:50 Click image to enlarge

CPTC-NME2-2 IHC NCI60

Result: Positive

Immunohistochemistry of CPTC-NME2-2 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:50


  IPMS
Download This PDF contains the spectra and recommendations for the use of this antibody in immuno capture mass spectrometry. (118.8 KB)

CPTC-NME2-2 Immuno-MS

Result: Positive

This PDF contains the spectra and recommendations for the use of this antibody in immuno capture mass spectrometry.


  Indirect ELISA
Click to enlarge image Indirect ELISA of CPTC-NME2-2 Click image to enlarge

CPTC-NME2-2 ELISA

Result: Positive

Indirect ELISA of CPTC-NME2-2


  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-NME2-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-NME2-2 Protein Array

Result: Positive

Protein Array in which CPTC-NME2-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot Using CPTC-NME2-2 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3). Click image to enlarge

CPTC-NME2-2 Western Blot

Result: Positive

Western Blot Using CPTC-NME2-2 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).


Background

Catalog Number:

CPTC-NME2-3

Target Antigen:

Nucleoside Diphosphate Kinase B

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

11/25/2019

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Click to enlarge image Kinetic titration data for NME2-3 Ab (606A21.1) using Biacore SPR method Click image to enlarge

CPTC-NME2-3 SPR

Result: Positive

Kinetic titration data for NME2-3 Ab (606A21.1) using Biacore SPR method


  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity between NME1 and NME2. Click image to enlarge

Cross Reactivity Studies

Result: Positive

This table shows the cross reactivity between NME1 and NME2.


Characterization SOP Files

  IPMS
Download This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry. (125.8 KB)

CPTC-NME2-3 Immuno-MS

Result: Negative

This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.


  Indirect ELISA
Click to enlarge image Indirect ELISA of CPTC-NME2-3 Click image to enlarge

CPTC-NME2-3 Indirect ELISA

Result: Positive

Indirect ELISA of CPTC-NME2-3


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot Using CPTC-NME2-3 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3). Click image to enlarge

CPTC-NME2-3 Western Blot

Result: Positive

Western Blot Using CPTC-NME2-3 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).


Background

NCI Identification Number:

10385

Antigen Name:

Nucleoside Diphosphate Kinase B

CPTC Name:

CPTC-NME2

Keywords:

Nucleoside Diphosphate Kinase B; NME2; NM23B; EC2.7.4.6; MGC111212; NDPKB; NM23-H2

Function:

Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC.

Acts as a transcriptional activator of the c-Myc gene; binds DNA nonspecifically

Chromosomal Localization:

17q21 - q22

Expression System:

E. Coli

NCBI Accession Number:

BC002476

Swiss Prot Accession Number:

P22392

DNA Source:

HIP:HsCD00002962

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

22523

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEH
LKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPA
DSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAH
DWVYE

Native Sequence:

MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQ
HYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSK
PGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWV
YE

Calculated Isoelectric Point:

8.52

Molecular Weight:

17570

Last Updated:

09/01/2008

Links

Characterization Data

Gel

Click to enlarge image Electrophoresis of antigen.  Lane 1 has molecular weight markers, lane 2 has the antigen.
Click image to enlarge

PAGE of ANL antigen 10385

Electrophoresis of antigen. Lane 1 has molecular weight markers, lane 2 has the antigen.

SOPs

Don't have Adobe Reader™?

Get it for free at Adobe.com