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Nucleoside Diphosphate Kinase B

Antibody: CPTC-NME2-1
Background

Catalog Number:

CPTC-NME2-1

RRID:

AB_1553805

Target Antigen:

Nucleoside Diphosphate Kinase B

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Click to enlarge image Kinetic titration data for NME2-1 Ab (606A1.1) using Biacore SPR method Click image to enlarge

CPTC-NME2-1 SPR

Result: Positive

Kinetic titration data for NME2-1 Ab (606A1.1) using Biacore SPR method


Characterization SOP Files

  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity between NME1 and NME2. Click image to enlarge

Cross Reactivity Studies

Result: Positive

This table shows the cross reactivity between NME1 and NME2.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (573.4 KB)

CPTC-NME2-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Download This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry. (123.0 KB)

CPTC-NME2-1 Immuno-MS

Result: Positive

This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (573.4 KB)

CPTC-NME2-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA of CPTC-NME2-1 Click image to enlarge

CPTC-NME2-1 ELISA

Result: Positive

Indirect ELISA of CPTC-NME2-1


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot Using CPTC-NME2-1 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3). Click image to enlarge

CPTC-NME2-1 Western Blot

Result: Positive

Western Blot Using CPTC-NME2-1 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).


Characterization SOP Files

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Antibody: CPTC-NME2-2
Background

Catalog Number:

CPTC-NME2-2

RRID:

AB_1553743

Target Antigen:

Nucleoside Diphosphate Kinase B

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Click to enlarge image Kinetic titration data for NME2-2 Ab (606A13.1) using Biacore SPR method Click image to enlarge

CPTC-NME2-2 SPR

Result: Positive

Kinetic titration data for NME2-2 Ab (606A13.1) using Biacore SPR method


Characterization SOP Files

  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity between NME1 and NME2. Click image to enlarge

Cross Reactivity Studies

Result: Positive

This table shows the cross reactivity between NME1 and NME2.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (548.6 KB)

CPTC-NME2-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Click to enlarge image Immunohistochemistry of CPTC-NME2-2 for NCI60 Cell Line Array. Data scored as: 0=NEGATIVE 1=WEAK (red) 2=MODERATE (blue) 3=STRONG (green) Titer: 1:50 Click image to enlarge

CPTC-NME2-2 IHC NCI60

Result: Positive

Immunohistochemistry of CPTC-NME2-2 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:50


Characterization SOP Files

  IP
Download This PDF contains the spectra and recommendations for the use of this antibody in immuno capture mass spectrometry. (118.8 KB)

CPTC-NME2-2 Immuno-MS

Result: Positive

This PDF contains the spectra and recommendations for the use of this antibody in immuno capture mass spectrometry.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (548.6 KB)

CPTC-NME2-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA of CPTC-NME2-2 Click image to enlarge

CPTC-NME2-2 ELISA

Result: Positive

Indirect ELISA of CPTC-NME2-2


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-NME2-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-NME2-2 Protein Array

Result: Positive

Protein Array in which CPTC-NME2-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot Using CPTC-NME2-2 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3). Click image to enlarge

CPTC-NME2-2 Western Blot

Result: Positive

Western Blot Using CPTC-NME2-2 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).


Characterization SOP Files

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Antibody: CPTC-NME2-3
Background

Catalog Number:

CPTC-NME2-3

RRID:

AB_1553741

Target Antigen:

Nucleoside Diphosphate Kinase B

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Click to enlarge image Kinetic titration data for NME2-3 Ab (606A21.1) using Biacore SPR method Click image to enlarge

CPTC-NME2-3 SPR

Result: Positive

Kinetic titration data for NME2-3 Ab (606A21.1) using Biacore SPR method


Characterization SOP Files

  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity between NME1 and NME2. Click image to enlarge

Cross Reactivity Studies

Result: Positive

This table shows the cross reactivity between NME1 and NME2.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (587.5 KB)

CPTC-NME2-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Download This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry. (125.8 KB)

CPTC-NME2-3 Immuno-MS

Result: Negative

This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (587.5 KB)

CPTC-NME2-3 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA of CPTC-NME2-3 Click image to enlarge

CPTC-NME2-3 Indirect ELISA

Result: Positive

Indirect ELISA of CPTC-NME2-3


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot Using CPTC-NME2-3 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3). Click image to enlarge

CPTC-NME2-3 Western Blot

Result: Positive

Western Blot Using CPTC-NME2-3 as primary Ab against Ag 10385(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).


Characterization SOP Files

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Antigen: Nucleoside Diphosphate Kinase B
Background

NCI Identification Number:

10385

Antigen Name:

Nucleoside Diphosphate Kinase B

CPTC Name:

CPTC-NME2

Aliases:

Nucleoside Diphosphate Kinase B; NME2; NM23B; EC2.7.4.6; MGC111212; NDPKB; NM23-H2

Function:

Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC.

Acts as a transcriptional activator of the c-Myc gene; binds DNA nonspecifically

Chromosomal Localization:

17q21 - q22

Expression System:

E. Coli

Accession Number:

BC002476

UniProt Accession Number:

P22392

DNA Source:

HIP:HsCD00002962

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

22523

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEH
LKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPA
DSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAH
DWVYE

Native Sequence:

MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQ
HYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSK
PGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWV
YE

Calculated Isoelectric Point:

8.52

Molecular Weight:

17570

Last Updated:

01/11/2021

Links

Characterization Data

Gel

Click to enlarge image Electrophoresis of antigen. Lane 1 has molecular weight markers, lane 2 has the antigen.
Click image to enlarge

PAGE of ANL antigen 10385

Electrophoresis of antigen. Lane 1 has molecular weight markers, lane 2 has the antigen.

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SOPs

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