Catalog Number:
CPTC-NEDD4-1
RRID:
AB_2617296
Target Antigen:
Neural precursor cell expressed, developmentally down-regulated 4 isoform 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-NEDD4-1 as primary Ab against NEDD4 (rAg 00011) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-NEDD4-2
RRID:
AB_2617297
Target Antigen:
Neural precursor cell expressed, developmentally down-regulated 4 isoform 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Human assay: HeLa fixed with PFA, dilution: 1:9
Human assay: MCF7 fixed with PFA, dilution: 1:9
Human assay: U-2 OS fixed with PFA, dilution: 1:9
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-NEDD4-2 as primary Ab against NEDD4 (rAg 00011) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-NEDD4-3
RRID:
AB_2617298
Target Antigen:
Neural precursor cell expressed, developmentally down-regulated 4 isoform 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-NEDD4-3 as primary Ab against NEDD4 (rAg 00011) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
NCI Identification Number:
00011
Antigen Name:
Neural precursor cell expressed, developmentally down-regulated 4 isoform 1
CPTC Name:
CPTC-NEDD4
Aliases:
neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitinprotein ligase; NEDD4-1; RPF1; KIAA0093; Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4; NEDD-4; MGC176705; neural precursor cell expressed, developmentally down-regulated 4; E3 ubiquitin-protein ligase NEDD4; receptor-potentiating factor 1; EC 6.3.2.
Function:
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a
thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the
degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor
internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN
regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates
TNK2 and regulates EGF-induced degradation of EGFR and TNF2.
Chromosomal Localization:
15q21.3
Expression System:
E. coli
Accession Number:
NM_006154
UniProt Accession Number:
P46934
DNA Source:
Mammalian Gene Collection
Immunogen:
Recombinant Full Length Protein
Vector Name:
pET28-MHL
Extinction Coefficient:
Buffers:
PBS, pH 7.4
Expressed Sequence:
mhhhhhhssgrenlyfqgMATCAVEVFGLLEDEENSRIVRVRVIAGIGLA
KKDILGASDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHP
QQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLH
PRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAAC
HLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAE
NGNIQLQAQRAFTTRRQISEETESVDNQESSENWEIIREDEATMYSSQAF
PSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAY
TFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQ
ATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHA
PNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEER
THTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRK
LKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKG
LDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHL
SYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYY
NSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEY
IYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGD
VDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSR
VPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEEL
WDKLQMAIENTQG
Native Sequence:
Calculated Isoelectric Point:
5.9
Molecular Weight:
105822
Last Updated:
08/22/2020
PAGE of NEDD4 (Ag 00011) with molecular weight standards in lane 1
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