Background
Catalog Number:
CPTC-MUC17 CRD1 L CRD2-1
RRID:
AB_2722093
Target Antigen:
Mucin 17 domain CRD1-L-CRD2
Isotype:
IgG2c
Species:
Mouse Monoclonal Antibody
Last Updated:
11/13/2019
Antigen Recognition(s):
Recombinant Full-length
SOP:
Purchase
Characterization Data [Compare Characterization Data]
Indirect ELISA
CPTC-MUC17 CRD1-L-CRD2 -1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-MUC17 CRD1-L-CRD2-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-MUC17 CRD1-L-CRD2-1 Western blot
Result: Positive
Western Blot using CPTC-MUC17-CRD1-L-CRD2-1 as primary Ab against MUC17-CRD1-L-CRD2 (rAg 00006) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-MUC17 CRD1 L CRD2-2
RRID:
AB_2617293
Target Antigen:
Mucin 17 domain CRD1-L-CRD2
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
11/13/2019
Antigen Recognition(s):
Recombinant Full-length
SOP:
Purchase
Characterization Data [Compare Characterization Data]
Indirect ELISA
CPTC-MUC17 CRD1-L-CRD2 -2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-MUC17 CRD1-L-CRD2-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-MUC17 CRD1-L-CRD2-2 Western blot
Result: Positive
Western Blot using CPTC-MUC17-CRD1-L-CRD2-2 as primary Ab against MUC17-CRD1-L-CRD2 (rAg 00006) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-MUC17 CRD1 L CRD2-3
RRID:
AB_2722094
Target Antigen:
Mucin 17 domain CRD1-L-CRD2
Isotype:
IgG2c
Species:
Mouse Monoclonal Antibody
Last Updated:
11/13/2019
Antigen Recognition(s):
Recombinant Full-length
SOP:
Purchase
Characterization Data [Compare Characterization Data]
Indirect ELISA
CPTC-MUC17 CRD1-L-CRD2 -3 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-MUC17 CRD1-L-CRD2-3 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-MUC17 CRD1-L-CRD2-3 Western blot
Result: Positive
Western Blot using CPTC-MUC17-CRD1-L-CRD2-3 as primary Ab against MUC17-CRD1-L-CRD2 (rAg 00006) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
00006
Antigen Name:
Mucin 17 domain CRD1-L-CRD2
CPTC Name:
CPTC-MUC17 CRD1-L-CRD2
Aliases:
intestinal membrane mucin MUC17; mucin 17; small intestinal mucin-3; secreted mucin MUC17; MUC-17; MUC3
Function:
MUC17 comprises of a signal peptide, a large central tandemly repeated central domain (TR), two EGF-like domains, a SEA (sea urchin sperm protein, enterokinase, and agrin) domain, a transmembrane domain (TM) and an 80 amino acid cytoplasmic tail (Cyt).
Human MUC17 and mouse homolog of human MUC17 i.e. Muc3 are highly expressed in the gallbladder, intestine, and colon and are localized in the upper villi of the intestine and on the surface and upper crypt cells of the colon. The expression of MUC17 is down regulated in ulcerative colitis (UC) and Crohn's disease (CD) as compared to normal controls. In addition to the inflammatory bowel disease (IBD), there is significant downregulation of MUC17 expression in the neoplastic conditions of colon such as hyperplastic polyps, tubular and tubulovillous adenoma and colon adenocarcinoma.
The extracellular regions contains two EGF-like Cys-rich domains (CRD1 and CRD2) connected by an intervening linker segment with SEA module (L), that may function to stimulate intestinal cell restitution.
Chromosomal Localization:
7q22
Expression System:
E.coli
Accession Number:
NM_001040105
UniProt Accession Number:
Q685J3
DNA Source:
MUC17-CRD1-L-CRD2 coding region was amplified from human intestinal cDNA
Immunogen:
Recombinant Domain
Vector Name:
pET28
Extinction Coefficient:
28780
Buffers:
50mM Tris (pH 8.2) buffer
Expressed Sequence:
RTTSRGCTTSASTLSATSTPHTSTSVTTRPVTPSSESSRPSTITSHTIPP
TFPPAHSSTPPTTSASSTTVNPEAVTTMTTRTKPSTRTTSFPTVTTTAVP
TNTTIKSNPTSTPTVPRTTTCFGDGCQNTASRCKNGGTWDGLKCQCPNLY
YGELCEEVVSSIDIGPPETISAQMELTVTVTSVKFTEELKNHSSQEFQEF
KQTFTEQMNIVYSGIPEYVGVNITKLRLGSVVVEHDVLLRTKYTPEYKTV
LDNATEVVKEKITKVTTQQIMINDICSDMMCFNTTGTQVQNITVTQYDPE
EDCRKMAKEYGDYFVVEYRDQKPYCISPCEPGFSVSKNCNLGKCQMSLSG
PQCLCVTTETHWYSGETCNQGTQKSV4390EHHHHHH
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
42062
Last Updated:
11/26/2012
Links
- Uniprot - Uniprot Q685J3
Characterization Data
SOPs
No SOPs available.
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