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Mucin 16 Peptide 3

Antibody: CPTC-MUC16-3
Background

Catalog Number:

CPTC-MUC16-3

Target Antigen:

Mucin 16 Peptide 3

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (510.1 KB)

CPTC-MUC16-3 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM screening of CPTC-MUC16-3 against synthetic peptide NIEDALNQLER (Mucin 16 Peptide 3) Data provided by the Carr Lab, Broad Institute https://www.broadinstitute.org/proteomics/protocols Click image to enlarge

CPTC-MUC16-3 iMRM (synthetic peptide)

Result: Positive

iMRM screening of CPTC-MUC16-3 against synthetic peptide NIEDALNQLER (Mucin 16 Peptide 3)

Data provided by the Carr Lab, Broad Institute
https://www.broadinstitute.org/proteomics/protocols


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (510.1 KB)

CPTC-MUC16-3 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-MUC16-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-MUC16-3 as primary antibody against recombinant human CA125/MUC16 protein (Met 13,360 ­- Gln 14,347). Expected molecular weight – 170 - 200 kDa. Molecular weight standards are also included (Lane 1). Blot was developed using enhanced chemiluminescence (ECL). Click image to enlarge

CPTC-MUC16-3 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-MUC16-3 as primary antibody against recombinant human CA125/MUC16 protein (Met 13,360 ­- Gln 14,347). Expected molecular weight – 170 - 200 kDa. Molecular weight standards are also included (Lane 1). Blot was developed using enhanced chemiluminescence (ECL).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MUC16-3 as primary antibody against cell culture supernatants OVCAR-3 (lane 2), OVCAR-4 (lane 3), OVCAR-8 (lane 4), and SK-OV-3 (lane 5) (Panel A) and against cell lysates OVCAR-3 (lane 2), OVCAR-4 (lane 3), OVCAR-8 (lane 4), and SK-OV-3 (lane 5) (Panel B). Molecular weight standards are also included in each panel (lane 1). Expected molecular weight > 250 kDa. Click image to enlarge

CPTC-MUC16-3 Western Blot Cell Lysates and Cell Culture Supernatants

Result: Negative

Western blot using CPTC-MUC16-3 as primary antibody against cell culture supernatants OVCAR-3 (lane 2), OVCAR-4 (lane 3), OVCAR-8 (lane 4), and SK-OV-3 (lane 5) (Panel A) and against cell lysates OVCAR-3 (lane 2), OVCAR-4 (lane 3), OVCAR-8 (lane 4), and SK-OV-3 (lane 5) (Panel B). Molecular weight standards are also included in each panel (lane 1). Expected molecular weight > 250 kDa.


Characterization SOP Files

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Antigen: Mucin 16 Peptide 3
Background

NCI Identification Number:

00481

Antigen Name:

Mucin 16 Peptide 3

CPTC Name:

CPTC-MUC16 Peptide 3

Aliases:

Mucin 16, Cell Surface Associated; CA125; Ovarian Cancer-Related Tumor Marker CA125; Ovarian Carcinoma Antigen CA125; Mucin-16; CA125 Ovarian Cancer Antigen; Mucin-16 Short Isoform; Mucin-16 Long Isoform; FLJ14303; MUC-16; CA-125; MUC16

Function:

This gene encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that play an important role in forming a protective mucous barrier, and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus, a large tandem repeat domain, and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated, while the repeat region contains 156 amino acid repeats unit that are rich in serines, threonines, and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules, leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain, and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier, protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers, with higher expression levels associated with poorer outcomes.MUC16 (Mucin 16, Cell Surface Associated) is a Protein Coding gene. Diseases associated with MUC16 include Ovarian Cyst and Clear Cell Adenocarcinoma. Among its related pathways are Defective C1GALT1C1 causes Tn polyagglutination syndrome (TNPS) and Metabolism of proteins.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

Chromosomal Localization:

19p13.2

Accession Number:

NP_078966.2

UniProt Accession Number:

Q8WXI7

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

NIEDALNQLFR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1210

Last Updated:

09/28/2022

Links

Characterization Data

SOPs

No SOPs available.

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