MutS Homolog 6


Background

Catalog Number:

CPTC-MSH6-1

RRID:

AB_2889966

Target Antigen:

MutS Homolog 6

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

06/23/2021

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (455.9 KB)

CPTC-MSH6-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Western Blot - Recombinant Protein or Peptide

CPTC-MSH6-1 Western Blot (recombinant protein)

Result: Negative

Western blot using CPTC-MSH6-1 as primary antibody against human mutS homolog 6 recombinant protein (lane 2). Expected molecular weight - 30.7 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

Background

NCI Identification Number:

00382

Antigen Name:

MutS Homolog 6

CPTC Name:

CPTC-MSH6 Peptide 1

Keywords:

MutS Homolog 6; DNA Mismatch Repair Protein Msh6; G/T Mismatch-Binding Protein; MutS-Alpha 160 KDa Subunit; MutS Protein Homolog 6; GTMBP; GTBP; P160; MutS (E. Coli) Homolog 6; MutS Homolog 6 (E. Coli); Sperm-Associated Protein; HNPCC5; HMSH6; HSAP; MSH6

Function:

This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described.MSH6 (MutS Homolog 6) is a Protein Coding gene. Diseases associated with MSH6 include Colorectal Cancer, Hereditary Nonpolyposis, Type 5 and Endometrial Cancer. Among its related pathways are Mismatch repair and Platinum Pathway, Pharmacokinetics/Pharmacodynamics. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and ATPase activity. An important paralog of this gene is MSH3.Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

Chromosomal Localization:

2p16.3

NCBI Accession Number:

NP_000170.1

UniProt Accession Number:

P52701

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LANLPEEVIQK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1210

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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