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MRE11 Homolog, Double Strand Break Repair Nuclease Peptide 2

Antibody: CPTC-MRE11A-2
Background

Catalog Number:

CPTC-MRE11A-2

Target Antigen:

MRE11 Homolog, Double Strand Break Repair Nuclease Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

05/15/2025

Antigen Recognition(s):

Peptide, Endogenous

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Characterization Data [Compare Characterization Data]
  Western Blot - Over-expressed transient protein in cell lysate

CPTC-MRE11A-2 Western Blot - Over-expressed transient protein in cell lysate

Result: Negative

Western blot using CPTC-MRE11A-2 as primary antibody against the over-expressed lysate of MRE11A. The antibody is not able to detect the target protein. Expected MW is 80.5 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-MRE11A-2 Western Blot - Cell Lysate

Result: Negative

Western blot using CPTC-MRE11A-2 as primary antibody against the whole cell lysates of HOP-92, SW-620, NCI-H226, T-47D, A549, and CAKI-1. The antibody is not able to detect the target protein in any of the tested cell lines.


Characterization SOP Files

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Antigen: MRE11 Homolog, Double Strand Break Repair Nuclease Peptide 2
Background

NCI Identification Number:

00502

Antigen Name:

MRE11 Homolog, Double Strand Break Repair Nuclease Peptide 2

CPTC Name:

CPTC-MRE11A Peptide 2

Aliases:

MRE11 Homolog, Double Strand Break Repair Nuclease; MRE11A; ATLD; MRE11 Homolog A, Double Strand Break Repair Nuclease; Double-Strand Break Repair Protein MRE11A; Double-Strand Break Repair Protein MRE11; Meiotic Recombination 11 Homolog 1; Meiotic Recombination 11 Homolog A; AT-Like Disease; MRE11 Homolog 1; HNGS1; MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae); MRE11 Homolog, Double Strand Break Repair Nuclease A; MRE11 Meiotic Recombination 11-Like Protein A; MRE11 Double Strand Break Repair Nuclease A; MRE11 Meiotic Recombination 11 Homolog A; DNA Recombination And Repair Protein; Endo/Exonuclease Mre11; MRE11 Homolog A; EC 3.1.-.-; MRE11B

Function:

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.MRE11 (MRE11 Homolog, Double Strand Break Repair Nuclease) is a Protein Coding gene. Diseases associated with MRE11 include Ataxia-Telangiectasia-Like Disorder 1 and Ataxia, Early-Onset, With Oculomotor Apraxia And Hypoalbuminemia. Among its related pathways are Cell Cycle Checkpoints and DNA Damage. Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11 (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). RAD50 may be required to bind DNA ends and hold them in close proximity (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289, PubMed:30612738). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation.

Chromosomal Localization:

11q21

Accession Number:

NP_005582.1

UniProt Accession Number:

P49959

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

NVQLSLLTER

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

08/31/2021

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Characterization Data

SOPs

No SOPs available.

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