MET Proto-Oncogene, Receptor Tyrosine Kinase


Background

Catalog Number:

CPTC-MET-1

Target Antigen:

MET Proto-Oncogene, Receptor Tyrosine Kinase

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

05/26/2016

Antigen Recognition(s):

Peptide, Phosphorylation

SOP:

Characterization Data [Compare Characterization Data]
  IPMS
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-MET-1 antibody with CPTC-MET peptide (NCI ID 00126) as the target antigen (sequence: MYDKEY(pY)SVHNK)
 Click image to enlarge

CPTC-MET-1 IPMS

Immuno-Precipitation Mass Spectrometry using CPTC-MET-1 antibody with CPTC-MET peptide (NCI ID 00126) as the target antigen (sequence: MYDKEY(pY)SVHNK)

  IPMS
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-MET-1 antibody with di-phospho MET peptide as the target antigen (sequence: MYDKE(pY)(pY)SVHNK) Click image to enlarge

CPTC-MET-1 IPMS cross reactivity

Immuno-Precipitation Mass Spectrometry using CPTC-MET-1 antibody with di-phospho MET peptide as the target antigen (sequence: MYDKE(pY)(pY)SVHNK)

  IPMS

CPTC-MET-1 IPMS cross reactivity

Immuno-Precipitation Mass Spectrometry using CPTC-MET-1 antibody with non-phospho MET peptide as the target antigen (sequence: MYDKEYYSVHNK. Results found that peptide was not recognized.

  IPMS

CPTC-MET-1 IPMS cross reactivity

Immuno-Precipitation Mass Spectrometry using CPTC-MET-1 antibody with mono-phospho MET peptide as the target antigen (sequence: MYDKE(pY)YSVHNK. Results found that peptide was not recognized.

Background

NCI Identification Number:

00126

Antigen Name:

MET Proto-Oncogene, Receptor Tyrosine Kinase

CPTC Name:

CPTC-MET

Keywords:

MET Proto-Oncogene, Receptor Tyrosine Kinase ; Tyrosine-Protein Kinase Met; Scatter Factor Receptor; Proto-Oncogene C-Met; HGF/SF Receptor; HGF Receptor; SF Receptor; EC 2.7.10.1; Met Proto-Oncogene Tyrosine Kinase; Hepatocyte Growth Factor Receptor; Soluble MET Variant 12; Met Proto-Oncogene; EC 2.7.10; DFNB97; AUTS9; RCCP2; c-MET; HGFR

Function:

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells
Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells.

The MET receptor, also known as hepatocyte growth factor receptor (HGFR), is a proto-oncogenic receptor tyrosine kinase. The endogenous ligand for MET is hepatocyte growth factor/scatter factor (HGF), a disulfide-linked heterodimeric molecule.

MET (MET Proto-Oncogene, Receptor Tyrosine Kinase) is a Protein Coding gene. Diseases associated with MET include renal cell carcinoma, papillary and hereditary papillary renal cell carcinoma. Among its related pathways are PI3K-Akt signaling pathway and GPCR Pathway. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is TYRO3.

Chromosomal Localization:

7q31

NCBI Accession Number:

NP_000236.2

Swiss Prot Accession Number:

P08581

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

PBS with 1% casein (or PBS with 1% BSA can also be used)

Expressed Sequence:

DKEY(pY)SVHNK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1100

Last Updated:

05/26/2016

Links

Characterization Data

SOPs

No SOPs available.

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