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Multiple Endocrine Neoplasia I

Antibody: CPTC-MEN1-1
Background

Catalog Number:

CPTC-MEN1-1

RRID:

AB_2617291

Target Antigen:

Multiple Endocrine Neoplasia I

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

08/23/2022

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (321.6 KB)

CPTC-MEN1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-MEN1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-MEN1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MEN1-1 as primary Ab against MEN1 (rAg 00058) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-MEN1-1 Western blot

Result: Positive

Western Blot using CPTC-MEN1-1 as primary Ab against MEN1 (rAg 00058) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Multiple Endocrine Neoplasia I
Background

NCI Identification Number:

00058

Antigen Name:

Multiple Endocrine Neoplasia I

CPTC Name:

CPTC-MEN1

Aliases:

SCG2; MEAI; Menin

Function:

Chromosomal Localization:

11q13

Expression System:

E. coli

Accession Number:

NP_000235.2

UniProt Accession Number:

O00255

DNA Source:

Mammalian Gene Collection

Immunogen:

Recombinant Full Length Protein

Vector Name:

pET28-MHL

Extinction Coefficient:

Buffers:

PBS pH 7.4

Expressed Sequence:

MHHHHHHSSGRENLYFQGMGLKAAQKTLFPLRSIDDVVRLFAAELGREEP
DLVLLSLVLGFVEHFLAVNRVIPTNVPELTFQPSPAPDPPGGLTYFPVAD
LSIIAALYARFTAQIRGAVDLSLYPREGGVSSRELVKKVSDVIWNSLSRS
YFKDRAHIQSLFSFITGTKLDSSGVAFAVVGACQALGLRDVHLALSEDHA
WVVFGPNGEQTAEVTWHGKGNEDRRGQTVNAGVAERSWLYLKGSYMRCDR
KMEVAFMVCAINPSIDLHTDSLELLQLQQKLLWLLYDLGHLERYPMALGN
LADLEELEPTPGRPDPLTLYHKGIASAKTYYRDEHIYPYMYLAGYHCRNR
NVREALQAWADTATVIQDYNYCREDEEIYKEFFEVANDVIPNLLKEAASL
LEAGEERPGEQSQGTQSQGSALQDPECFAHLLRFYDGICKWEEGSPTPVL
HVGWATFLVQSLGRFEGQVRQKVRIVSREAEAAEAEEPWGEEAREGRRRG
PRRESKPEEPPPPKKPALDKGLGTGQGAVSGP

Native Sequence:

Calculated Isoelectric Point:

5.6

Molecular Weight:

59520

Last Updated:

08/22/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of MEN1 (rAg 00058) with molecular weight standards in lane 1
Click image to enlarge

CPTC-MEN1 PAGE

PAGE of MEN1 (rAg 00058) with molecular weight standards in lane 1

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SOPs

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