Catalog Number:
CPTC-MDC1-1
RRID:
AB_2722082
Target Antigen:
Mediator Of DNA-Damage Checkpoint 1 Peptide 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/01/2022
Antigen Recognition(s):
Peptide, Endogenous, Phosphorylation
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-1 antibody with CPTC-MDC1 peptide 1 (phosphorylated) as the target antigen.
Result: Positive
Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-1 antibody with CPTC-MDC1 peptide 1 (non-phosphorylated) as the target antigen.
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Positive
Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Catalog Number:
CPTC-MDC1-2
RRID:
AB_2722083
Target Antigen:
Mediator Of DNA-Damage Checkpoint 1 Peptide 1
Isotype:
IgG3
Species:
Mouse Monoclonal Antibody
Last Updated:
09/01/2022
Antigen Recognition(s):
Peptide, Phosphorylation
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-1 antibody with CPTC-MDC1 peptide 1 (phosphorylated) as the target antigen.
Result: Positive
Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-1 antibody with CPTC-MDC1 peptide 1 (non-phosphorylated) as the target antigen.
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Positive
Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Catalog Number:
CPTC-MDC1-3
RRID:
AB_2722084
Target Antigen:
Mediator Of DNA-Damage Checkpoint 1 Peptide 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/01/2022
Antigen Recognition(s):
Peptide, Phosphorylation
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-3 antibody with CPTC-MDC1 peptide 1 (phosphorylated) as the target antigen.
Result: Positive
Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-3 antibody with CPTC-MDC1 peptide 1 (non-phosphorylated) as the target antigen.
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Positive
Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Catalog Number:
CPTC-MDC1-4
RRID:
AB_2820266
Target Antigen:
Mediator Of DNA-Damage Checkpoint 1 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
03/20/2024
Antigen Recognition(s):
Peptide, Endogenous, Phosphorylation
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
mmuno-MRM chromatogram of CPTC-MDC1-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3235)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)
Result: Positive
Immuno-MRM chromatogram of CPTC-MDC1-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3235)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Catalog Number:
CPTC-MDC1-5
Target Antigen:
Mediator Of DNA-Damage Checkpoint 1 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
09/01/2022
Antigen Recognition(s):
Peptide, Phosphorylation
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
mmuno-MRM chromatogram of CPTC-MDC1-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3234)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
NCI Identification Number:
00100
Antigen Name:
Mediator Of DNA-Damage Checkpoint 1 Peptide 1
CPTC Name:
CPTC-MDC1 Peptide 1
Aliases:
NFBD1; Nuclear Factor With BRCT Domains 1; KIAA0170; Homologue To Drosophila Photoreceptor Protein Calphotin; Mediator Of DNA Damage Checkpoint 1; Mediator Of DNA Damage Checkpoint Protein 1
Function:
The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a
central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
xMDC1 (mediator of DNA-damage checkpoint 1) is a protein-coding gene. GO annotations related to this gene include protein C-terminus binding and FHA domain binding. An important paralog of this gene is PAXIP1.
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
Chromosomal Localization:
6p21.3
Accession Number:
NP_055456.2
UniProt Accession Number:
Q14676
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
AQPFGFID(pS)DTDAEEER (pS329)
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1870
Last Updated:
03/11/2015
No SOPs available.
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