Mitogen-Activated Protein Kinase 8 Peptide 1


Background

Catalog Number:

CPTC-MAPK8-1

Target Antigen:

Mitogen-Activated Protein Kinase 8 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

11/21/2023

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (355.0 KB)

CPTC-MAPK8-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Immunofluorescence

CPTC-MAPK8-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of HeLa and MCF7 cells using CPTC-MAPK8-1 antibody. MAPK8 protein expression was not detected in either cell lines.


  Western Blot - Recombinant Protein or Peptide

CPTC-MAPK8-1 Western Blot (Recombinant Protein)

Result: Negative

Western blot using CPTC-MAPK8-1 as primary antibody against human mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-b1 recombinant protein (lane 2). Molecular weight standards are also included (lane 1). Expected molecular weight – 43.8 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-MAPK8-1 as primary antibody against cell lysates A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF-7 (lane 5), MOLT-4 (lane 6), and U87 (lane 7). Molecular weight standards are also included (lane 1). All cell lines are positive. Expected molecular weights – 48.3 kDa, 44.2 kDa, 44.0 kDa, 48.1 kDa, and 35.3 kDa. Click image to enlarge

CPTC-MAPK8-1 Western Blot Cell Lysates

Result: Positive

Western Blot using CPTC-MAPK8-1 as primary antibody against cell lysates A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF-7 (lane 5), MOLT-4 (lane 6), and U87 (lane 7). Molecular weight standards are also included (lane 1). All cell lines are positive. Expected molecular weights – 48.3 kDa, 44.2 kDa, 44.0 kDa, 48.1 kDa, and 35.3 kDa.


Characterization SOP Files

Background

NCI Identification Number:

00459

Antigen Name:

Mitogen-Activated Protein Kinase 8 Peptide 1

CPTC Name:

CPTC-MAPK8 Peptide 1

Aliases:

Mitogen-Activated Protein Kinase 8; SAPK1; JNK1; Stress-Activated Protein Kinase 1c; C-Jun N-Terminal Kinase 1; JUN N-Terminal Kinase; MAP Kinase 8; EC 2.7.11.24; JNK-46; SAPK1c; PRKM8; JNK; Mitogen-Activated Protein Kinase 8 Isoform JNK1 Alpha1; Mitogen-Activated Protein Kinase 8 Isoform JNK1 Beta2; Stress-Activated Protein Kinase JNK1; Stress-Activated Protein Kinase 1; JNK21B1/2; EC 2.7.11; JNK1A2; SAPK1C; MAPK 8; MAPK8

Function:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. MAPK8 (Mitogen-Activated Protein Kinase 8) is a Protein Coding gene. Diseases associated with MAPK8 include Fatty Liver Disease and Hepatitis C. Among its related pathways are ATM Pathway and Association Between Physico-Chemical Features and Toxicity Associated Pathways. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is MAPK10.Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (By similarity). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity).NK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Chromosomal Localization:

10q11.22

Accession Number:

NP_001265476.1

UniProt Accession Number:

P45983

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

TAGTSFMMT(ph)PY(ph)VVTR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1650

Last Updated:

10/23/2020

Links

Characterization Data

SOPs

No SOPs available.

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