Background
Catalog Number:
CPTC-MAP2K2-1
Target Antigen:
Mitogen-Activated Protein Kinase Kinase 2 Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
08/23/2022
Antigen Recognition(s):
Peptide, Recombinant Full-length
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (342.1 KB)
CPTC-MAP2K2-1 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-MAP2K2-1 Western Blot (Recombinant Protein)
Result: Positive
Western blot using CPTC-MAP2K2-1 as primary antibody against human mitogen-activated protein kinase kinase 2 (MAP2K2) recombinant protein (lane 2). Molecular weight standards are also included (lane 1). Expected molecular weight – 44.2 kDa. Blot was developed using enhanced chemiluminescence (ECL).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-MAP2K2-1 Western Blot Cell Lysates
Result: Negative
Western Blot using CPTC-MTOR-10 as primary antibody against cell lysates A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF-7 (lane 5), MOLT-4 (lane 6), and U87 (lane 7). Molecular weight standards are also included (lane 1). All cell lines are negative. Expected molecular weights – 44.4 kDa.
Characterization SOP Files
Background
NCI Identification Number:
00461
Antigen Name:
Mitogen-Activated Protein Kinase Kinase 2 Peptide 1
CPTC Name:
CPTC-MAP2K2 Peptide 1
Aliases:
Mitogen-Activated Protein Kinase Kinase 2; MEK2; Dual Specificity Mitogen-Activated Protein Kinase Kinase 2; ERK Activator Kinase 2; MAP Kinase Kinase 2; MAPK/ERK Kinase 2; EC 2.7.12.2; PRKMK2; MKK2; Mitogen-Activated Protein Kinase Kinase 2, P45; MAPKK 2; MAPKK2; MAP2K2; MEK 2; CFC4
Function:
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. MAP2K2 (Mitogen-Activated Protein Kinase Kinase 2) is a Protein Coding gene. Diseases associated with MAP2K2 include Cardiofaciocutaneous Syndrome 4 and Cardiofaciocutaneous Syndrome 1. Among its related pathways are Common Cytokine Receptor Gamma-Chain Family Signaling Pathways and Negative regulation of MAPK pathway. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is ENSG00000230626.Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation
Chromosomal Localization:
19p13.3
Accession Number:
NP_109587.1
UniProt Accession Number:
P36507
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
LCDFGVSGQLIDS(ph)MANSFVGTR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
2420
Last Updated:
11/19/2020
Links
- EnsEMBL - EnsEMBL MAP2K2
- Entrez - Entrez MAP2K2
- Uniprot - Uniprot P36507
Characterization Data
SOPs
No SOPs available.
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