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Lymphocyte Antigen 6 Family Member E

Antibody: CPTC-Ly6E-1
Background

Catalog Number:

CPTC-Ly6E-1

Target Antigen:

Lymphocyte Antigen 6 Family Member E

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2023

Antigen Recognition(s):

Recombinant Full-length

Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR

CPTC-LY6E-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Negative

Affinity and binding kinetics of CPTC-LY6E-1 and full length LY6E recombinant protein were measured using surface plasmon resonance. LY6E recombinant protein was amine coupled onto a Series S CM5 chip. CPTC-LY6E-1 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a bivalent fitting model.


Characterization SOP Files

  Indirect ELISA

CPTC-Ly6E-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-Ly6E-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-Ly6E-1 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-Ly6E-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

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Antibody: CPTC-Ly6E-2
Background

Catalog Number:

CPTC-Ly6E-2

Target Antigen:

Lymphocyte Antigen 6 Family Member E

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Recombinant Full-length

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Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR

CPTC-LY6E-2 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Negative

Affinity and binding kinetics of CPTC-LY6E-2 and full length LY6E recombinant protein were measured using surface plasmon resonance. LY6E recombinant protein was amine coupled onto a Series S CM5 chip. CPTC-LY6E-2 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a bivalent fitting model.


Characterization SOP Files

  Indirect ELISA

CPTC-LY6E-2 Indirect ELISA

Result: Negative

Indirect ELISA using CPTC-LY6E-2 as primary mouse antibody against full length LY6E recombinant protein. Recombinant protein was coated on the plate and CPTC-LY6E-2 antibody was titrated and detected using the goat anti-mouse antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-Ly6E-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

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Antibody: CPTC-Ly6E-3
Background

Catalog Number:

CPTC-Ly6E-3

Target Antigen:

Lymphocyte Antigen 6 Family Member E

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR

CPTC-LY6E-3 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Negative

Affinity and binding kinetics of CPTC-LY6E-3 and full length LY6E recombinant protein were measured using surface plasmon resonance. LY6E recombinant protein was amine coupled onto a Series S CM5 chip. CPTC-LY6E-3 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a bivalent fitting model.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-Ly6E-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

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Antigen: Lymphocyte Antigen 6 Family Member E
Background

NCI Identification Number:

00233

Antigen Name:

Lymphocyte Antigen 6 Family Member E

CPTC Name:

CPTC-Ly6E

Aliases:

Lymphocyte Antigen 6 Family Member E; Lymphocyte Antigen 6 Complex, Locus E; Retinoic Acid-Induced Gene E Protein; Retinoic Acid Induced Gene E; Thymic Shared Antigen 1; Stem Cell Antigen 2; Ly-6E; RIG-E; SCA-2; TSA-1; SCA2; RIGE; Lymphocyte Antigen 6E; 9804; TSA1

Function:

LY6E (Lymphocyte Antigen 6 Family Member E) is a Protein Coding gene. Diseases associated with LY6E include Leukemia, Acute Promyelocytic, Somatic. Among its related pathways are Ectoderm Differentiation and Metabolism of proteins.

Involved in T-cell development. Believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits alpha-3:beta-4-containing nAChRs maximum response.

Chromosomal Localization:

8q24.3

Expression System:

E.coli

Accession Number:

NP_001120685.1

UniProt Accession Number:

Q16553

DNA Source:

N/A

Immunogen:

Recombinant Full Length Protein

Vector Name:

pET 28 HA tag

Extinction Coefficient:

Buffers:

0.5% Triton buffer

Expressed Sequence:

MRTALLLLATLAVATGPALTLRCHVCTSSSNCKHSVVCPASSRFCKTTNT
VEPLRGNLVKKDCAESCTPSYTLQGQVSSGTSSTQCCQEDLCNEKLHNAA
PTRTALAHSALSLGLALSLLAVILAPSL

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

14400

Last Updated:

02/13/2018

Links

Characterization Data

SOPs

No SOPs available.

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