Catalog Number:
CPTC-Ly6E-1
Target Antigen:
Lymphocyte Antigen 6 Family Member E
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2023
Antigen Recognition(s):
Recombinant Full-length
Result: No Binding
Affinity and binding kinetics of CPTC-LY6E-1 and full length LY6E recombinant protein were measured using surface plasmon resonance. LY6E recombinant protein was amine coupled onto a Series S CM5 chip. CPTC-LY6E-1 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a bivalent fitting model.
Result: High Binding
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Positive
Protein Array in which CPTC-Ly6E-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Catalog Number:
CPTC-Ly6E-2
Target Antigen:
Lymphocyte Antigen 6 Family Member E
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
02/09/2024
Antigen Recognition(s):
Recombinant Full-length
Result: No Binding
Affinity and binding kinetics of CPTC-LY6E-2 and full length LY6E recombinant protein were measured using surface plasmon resonance. LY6E recombinant protein was amine coupled onto a Series S CM5 chip. CPTC-LY6E-2 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a bivalent fitting model.
Result: No Binding
Indirect ELISA using CPTC-LY6E-2 as primary mouse antibody against full length LY6E recombinant protein. Recombinant protein was coated on the plate and CPTC-LY6E-2 antibody was titrated and detected using the goat anti-mouse antibody and TMB.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Catalog Number:
CPTC-Ly6E-3
Target Antigen:
Lymphocyte Antigen 6 Family Member E
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
02/09/2024
Antigen Recognition(s):
Recombinant Full-length
Result: No Binding
Affinity and binding kinetics of CPTC-LY6E-3 and full length LY6E recombinant protein were measured using surface plasmon resonance. LY6E recombinant protein was amine coupled onto a Series S CM5 chip. CPTC-LY6E-3 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM, and 0.0625 nM. All binding data were double-referenced and analyzed globally using a bivalent fitting model.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
NCI Identification Number:
00233
Antigen Name:
Lymphocyte Antigen 6 Family Member E
CPTC Name:
CPTC-Ly6E
Aliases:
Lymphocyte Antigen 6 Family Member E; Lymphocyte Antigen 6 Complex, Locus E; Retinoic Acid-Induced Gene E Protein; Retinoic Acid Induced Gene E; Thymic Shared Antigen 1; Stem Cell Antigen 2; Ly-6E; RIG-E; SCA-2; TSA-1; SCA2; RIGE; Lymphocyte Antigen 6E; 9804; TSA1
Function:
LY6E (Lymphocyte Antigen 6 Family Member E) is a Protein Coding gene. Diseases associated with LY6E include Leukemia, Acute Promyelocytic, Somatic. Among its related pathways are Ectoderm Differentiation and Metabolism of proteins.
Involved in T-cell development. Believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits alpha-3:beta-4-containing nAChRs maximum response.
Chromosomal Localization:
8q24.3
Expression System:
E.coli
Accession Number:
NP_001120685.1
UniProt Accession Number:
Q16553
DNA Source:
N/A
Immunogen:
Recombinant Full Length Protein
Vector Name:
pET 28 HA tag
Extinction Coefficient:
Buffers:
0.5% Triton buffer
Expressed Sequence:
MRTALLLLATLAVATGPALTLRCHVCTSSSNCKHSVVCPASSRFCKTTNT
VEPLRGNLVKKDCAESCTPSYTLQGQVSSGTSSTQCCQEDLCNEKLHNAA
PTRTALAHSALSLGLALSLLAVILAPSL
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
14400
Last Updated:
02/13/2018
No SOPs available.
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