Background
Catalog Number:
CPTC-LIG3-1
Target Antigen:
DNA Ligase 3 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
02/06/2024
Antigen Recognition(s):
Peptide, Endogenous
Purchase
- DSHB CPTC-LIG3-1
External Links
- Human Protein Atlas CPTC-LIG3-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (442.6 KB)
CPTC-LIG3-1 evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immunofluorescence - HPA
CPTC-LIG3-1 evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org).
The subcellular location is supported by literature.
Immunofluorescent staining of human cell line THP-1 shows localization to nucleoplasm.
Human assay: THP-1 fixed with PFA, dilution: 1:188
Human assay: U2OS fixed with PFA, dilution: 1:188
Characterization SOP Files
Background
NCI Identification Number:
00425
Antigen Name:
DNA Ligase 3 Peptide 1
CPTC Name:
CPTC-LIG3 Peptide 1
Aliases:
DNA Ligase 3; Polydeoxyribonucleotide Synthase [ATP] 3; Ligase III, DNA, ATP-Dependent; Ligase II, DNA, ATP-Dependent; EC 6.5.1.1; LIG3alpha; DNA Ligase III; LIG2; LIG3
Function:
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.LIG3 (DNA Ligase 3) is a Protein Coding gene. Diseases associated with LIG3 include Bloom Syndrome and Vaginal Carcinosarcoma. Among its related pathways are Transcription-Coupled Nucleotide Excision Repair (TC-NER) and Telomere C-strand (Lagging Strand) Synthesis. Gene Ontology (GO) annotations related to this gene include DNA ligase activity and DNA ligase (ATP) activity. An important paralog of this gene is LIG1.Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627).Ligases are enzymes which catalyze the joining of two molecules with concomitant hydrolysis of the diphosphate bond in ATP or a similar triphosphate. They belong to the E.C. 6 class of enzymes, which also includes synthases and carboxylases.
Chromosomal Localization:
17q12
Accession Number:
NP_039269.2
UniProt Accession Number:
P49916
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
VIQEGLEGLVLK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1320
Last Updated:
09/20/2020
Links
- EnsEMBL - EnsEMBL LIG3
- Entrez - Entrez LIG3
- Uniprot - Uniprot P49916
Characterization Data
SOPs
No SOPs available.
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