LINE1 Retrotransposable Element 1 Peptide 2


Background

Catalog Number:

CPTC-L1RE1-2

Target Antigen:

LINE1 Retrotransposable Element 1 Peptide 2

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide

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Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image iMRM screening of CPTC-L1RE1-2 against synthetic peptide LIGVPESDVENGTK (LINE1 Retrotransposable Element 1 Peptide 2)

Data provided by the Carr Lab, Broad Institute
https://www.broadinstitute.org/proteomics/protocols Click image to enlarge

CPTC-L1RE1-2 iMRM (synthetic peptide)

Result: Positive

iMRM screening of CPTC-L1RE1-2 against synthetic peptide LIGVPESDVENGTK (LINE1 Retrotransposable Element 1 Peptide 2)

Data provided by the Carr Lab, Broad Institute
https://www.broadinstitute.org/proteomics/protocols


  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-L1RE1-2 as primary antibody against  cell lysates OVCAR-3 (lane 2), OVCAR-4 (lane 3), OVCAR-8 (lane 4), and SK-OV-3 (lane 5). Molecular weight standards are also included (lane 1). Expected molecular weight 40KDa. Click image to enlarge

CPTC-L1RE1-2 Western Blot (Lysates)

Result: Negative

Western blot using CPTC-L1RE1-2 as primary antibody against cell lysates OVCAR-3 (lane 2), OVCAR-4 (lane 3), OVCAR-8 (lane 4), and SK-OV-3 (lane 5). Molecular weight standards are also included (lane 1). Expected molecular weight 40KDa.


Characterization SOP Files

Background

NCI Identification Number:

00417

Antigen Name:

LINE1 Retrotransposable Element 1 Peptide 2

CPTC Name:

CPTC-L1RE1 Peptide 2

Aliases:

LINE1 Retrotransposable Element 1; LINE Retrotransposable Element 1; LRE1

Function:

Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome.May function as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing thetranscript from which they are encoded.

Chromosomal Localization:

22q11.1-q11.2

Accession Number:

x

UniProt Accession Number:

Q9UN81

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LIGVPESDVENGTK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1540

Last Updated:

09/20/2020

Links

Characterization Data

SOPs

No SOPs available.

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