Background
Catalog Number:
CPTC-L1RE1-1
Target Antigen:
LINE1 Retrotransposable Element 1 Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
02/06/2024
Antigen Recognition(s):
Peptide, Endogenous
Purchase
- DSHB CPTC-L1RE1-1
Characterization Data [Compare Characterization Data]
Immuno-MRM
CPTC-L1RE1-1 iMRM screening (synthetic peptide)
Result: Positive
iMRM screening of CPTC-L1RE1-1 against synthetic peptide LSFISEGEIK (LINE1 Retrotransposable Element 1 Peptide 1)
Data provided by the Carr Lab, Broad Institute
https://www.broadinstitute.org/proteomics/protocols
Characterization SOP Files
Immunofluorescence
CPTC-L1RE1-1 Immunofluorescence
Result: Negative
Immunofluorescence staining of human cell lines OVCAR-3, OVCAR-8 and SK-OV-3 with CPTC-L1RE1-1 Ab shows no localization of L1RE1 protein.
Characterization SOP Files
NCI 60 Protein Array
CPTC-L1RE1-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-L1RE1-1 Western Blot (Lysates)
Result: Presumed Positive (with additional bands)
Western blot using CPTC-L1RE1-1 as primary antibody against cell lysates OVCAR-3 (lane 2), OVCAR-4 (lane 3), OVCAR-8 (lane 4), and SK-OV-3 (lane 5). Molecular weight standards are also included (lane 1). Expected molecular weight 40KDa.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-L1RE1-1 Single Cell Western Blot (Cell Lysate)
Result: Negative
Single cell western blot using CPTC-L1RE1-1 as a primary antibody against OVCAR-3, OVCAR-8 and SK-OV-3 cell lysates. L1RE1 protein expression was not detected.
Characterization SOP Files
Background
NCI Identification Number:
00416
Antigen Name:
LINE1 Retrotransposable Element 1 Peptide 1
CPTC Name:
CPTC-L1RE1 Peptide 1
Aliases:
LINE1 Retrotransposable Element 1; LINE Retrotransposable Element 1; LRE1
Function:
Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome.May function as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing thetranscript from which they are encoded.
Chromosomal Localization:
22q11.1-q11.2
Accession Number:
X
UniProt Accession Number:
Q9UN81
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
LSFISEGEIK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1100
Last Updated:
09/20/2020
Links
- Uniprot - Uniprot Q9UN81
Characterization Data
SOPs
No SOPs available.
Don't have Adobe Reader™?
Get it for free at Adobe.com