Background
Catalog Number:
CPTC-KIAA1967-1
RRID:
AB_2617284
Target Antigen:
KIAA1967
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/03/2026
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-KIAA1967-1
External Links
- Human Protein Atlas CPTC-KIAA1967-1
Characterization Data [Compare Characterization Data]
CyTOF
CPTC-KIAA1967-1 Immuno-Mass Cytometry
Result: Positive
Imaging mass cytometry on colon cancer tissue core using CPTC-KIAA1967-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, prostate, liver, colon, endometrium, appendix, pancreas, bone marrow, breast, kidney, lung, and placenta) and cancer tissues (breast, lung, prostate, colon and ovarian).
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (164.1 KB)
CPTC-KIAA1967-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Immunofluorescence - HPA
CPTC-KIAA1967-1 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000
Characterization SOP Files
Indirect ELISA
CPTC-KIAA1967-1 Indirect ELISA
Result: High Binding
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-KIAA1967-1 NCI 60 Protein Array
Result: Positive
Protein Array in which CPTC-KIAA1967-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-KIAA1967-1 Western blot
Result: Positive
Western Blot using CPTC-KIAA1967-1 as primary Ab against KIAA1967 (rAg 00056) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-KIAA1967-2
RRID:
AB_2617285
Target Antigen:
KIAA1967
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-KIAA1967-2
External Links
- Human Protein Atlas CPTC-KIAA1967-2
Characterization Data [Compare Characterization Data]
Immunofluorescence - HPA
CPTC-KIAA1967-2 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & nuclear bodies. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000
Characterization SOP Files
Indirect ELISA
CPTC-KIAA1967-2 Indirect ELISA
Result: High Binding
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-KIAA1967-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-KIAA1967-2 Western blot
Result: Positive
Western Blot using CPTC-KIAA1967-2 as primary Ab against KIAA1967 (rAg 00056) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
00056
Antigen Name:
KIAA1967
CPTC Name:
CPTC-KIAA1967
Aliases:
DBC1; DBC-1; NET35; P30 DBC; DBIRD Complex Subunit KIAA1967; Deleted In Breast Cancer 1; P30 DBC Protein; P30DBC; DBC.1; Deleted In Breast Cancer Gene 1 Protein
Function:
Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity
Chromosomal Localization:
8p22
Expression System:
E. coli
Accession Number:
NP_066997.3
UniProt Accession Number:
Q8N163
DNA Source:
Mammalian Gene Collection
Immunogen:
Recombinant Full Length Protein
Vector Name:
pET28-MHL
Extinction Coefficient:
Buffers:
PBS pH 7.4
Expressed Sequence:
MHHHHHHSSGRENLYFQGKKRKQRAGGEPWGAKKPRHDLPPYRVHLTPYT
VDSPICDFLELQRRYRSLLVPSDFLSVHLSWLSAFPLSQPFSLHHPSRIQ
VSSEKEAAPDAGAEPITADSDPAYSSKVLLLSSPGLEELYRCCMLFVDDM
AEPRETPEHPLKQIKFLLGRKEEEAVLVGGEWSPSLDGLDPQADPQVLVR
TAIRCAQAQTGIDLSGCTKWWRFAEFQYLQPGPPRRLQTVVVYLPDVWTI
MPTLEEWEALCQQKAAEAAPPTQEAQGETEPTEQAPDALEQAADTSRRNA
ETPEATTQQETDTDLPEAPPPPLEPAVIARPGCVNLSLHGIVEDRRPKER
ISFEVMVLAELFLEMLQRDFGYRVYKMLLSLPEKVVSPPEPEKEEAAKEE
ATKEEEAIKEEVVKEPKDEAQNEGPATES
Native Sequence:
Calculated Isoelectric Point:
4.7
Molecular Weight:
48265
Last Updated:
10/23/2013
Links
- EnsEMBL - EnsEMBL KIAA1967
- Entrez - Entrez KIAA1967
- Uniprot - Uniprot Q8N163
Characterization Data
Gel
Click image to enlarge
CPTC-KIAA1967 PAGE
PAGE of KIAA1967 (rAg 00056) with molecular weight standards in lane 1
Characterization SOP Files
SOPs
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