Background
Catalog Number:
CPTC-KIAA1967-1
RRID:
AB_2617284
Target Antigen:
KIAA1967
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-KIAA1967-1
External Links
- Human Protein Atlas CPTC-KIAA1967-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (164.1 KB)
CPTC-KIAA1967-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Immunofluorescence - HPA
CPTC-KIAA1967-1 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000
Characterization SOP Files
Indirect ELISA
CPTC-KIAA1967-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-KIAA1967-1 NCI 60 Protein Array
Result: Positive
Protein Array in which CPTC-KIAA1967-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-KIAA1967-1 Western blot
Result: Positive
Western Blot using CPTC-KIAA1967-1 as primary Ab against KIAA1967 (rAg 00056) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-KIAA1967-2
RRID:
AB_2617285
Target Antigen:
KIAA1967
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-KIAA1967-2
External Links
- Human Protein Atlas CPTC-KIAA1967-2
Characterization Data [Compare Characterization Data]
Immunofluorescence - HPA
CPTC-KIAA1967-2 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & nuclear bodies. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000
Characterization SOP Files
Indirect ELISA
CPTC-KIAA1967-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-KIAA1967-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-KIAA1967-2 Western blot
Result: Positive
Western Blot using CPTC-KIAA1967-2 as primary Ab against KIAA1967 (rAg 00056) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
00056
Antigen Name:
KIAA1967
CPTC Name:
CPTC-KIAA1967
Aliases:
DBC1; DBC-1; NET35; P30 DBC; DBIRD Complex Subunit KIAA1967; Deleted In Breast Cancer 1; P30 DBC Protein; P30DBC; DBC.1; Deleted In Breast Cancer Gene 1 Protein
Function:
Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity
Chromosomal Localization:
8p22
Expression System:
E. coli
Accession Number:
NP_066997.3
UniProt Accession Number:
Q8N163
DNA Source:
Mammalian Gene Collection
Immunogen:
Recombinant Full Length Protein
Vector Name:
pET28-MHL
Extinction Coefficient:
Buffers:
PBS pH 7.4
Expressed Sequence:
MHHHHHHSSGRENLYFQGKKRKQRAGGEPWGAKKPRHDLPPYRVHLTPYT
VDSPICDFLELQRRYRSLLVPSDFLSVHLSWLSAFPLSQPFSLHHPSRIQ
VSSEKEAAPDAGAEPITADSDPAYSSKVLLLSSPGLEELYRCCMLFVDDM
AEPRETPEHPLKQIKFLLGRKEEEAVLVGGEWSPSLDGLDPQADPQVLVR
TAIRCAQAQTGIDLSGCTKWWRFAEFQYLQPGPPRRLQTVVVYLPDVWTI
MPTLEEWEALCQQKAAEAAPPTQEAQGETEPTEQAPDALEQAADTSRRNA
ETPEATTQQETDTDLPEAPPPPLEPAVIARPGCVNLSLHGIVEDRRPKER
ISFEVMVLAELFLEMLQRDFGYRVYKMLLSLPEKVVSPPEPEKEEAAKEE
ATKEEEAIKEEVVKEPKDEAQNEGPATES
Native Sequence:
Calculated Isoelectric Point:
4.7
Molecular Weight:
48265
Last Updated:
10/23/2013
Links
- EnsEMBL - EnsEMBL KIAA1967
- Entrez - Entrez KIAA1967
- Uniprot - Uniprot Q8N163
Characterization Data
Gel
Click image to enlarge
CPTC-KIAA1967 PAGE
PAGE of KIAA1967 (rAg 00056) with molecular weight standards in lane 1
Characterization SOP Files
SOPs
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