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Indoleamine 2,3-Dioxygenase 1

Antibody: CPTC-IDO1-1
Background

Catalog Number:

CPTC-IDO1-1

RRID:

AB_2617275

Target Antigen:

Indoleamine 2,3-Dioxygenase 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2021

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-IDO1-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-IDO1-1 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-IDO1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-IDO1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-IDO1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-IDO1-1 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-IDO1-1 Western Blot

Result: Positive

Western Blot using CPTC-IDO1-1 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-IDO1-2
Background

Catalog Number:

CPTC-IDO1-2

RRID:

AB_2617276

Target Antigen:

Indoleamine 2,3-Dioxygenase 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

07/28/2021

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (213.4 KB)

CPTC-IDO1-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-IDO1-2 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-IDO1-2. Titer: 1:8000 Click image to enlarge

CPTC-IDO1-2 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-IDO1-2. Titer: 1:8000


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-IDO1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-IDO1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-IDO1-2 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-IDO1-2 Western Blot

Result: Positive

Western Blot using CPTC-IDO1-2 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Indoleamine 2,3-Dioxygenase 1
Background

NCI Identification Number:

10898

Antigen Name:

Indoleamine 2,3-Dioxygenase 1

CPTC Name:

CPTC-IDO1

Aliases:

Indoleamine 2,3-Dioxygenase 1; Indoleamine-Pyrrole 2,3-Dioxygenase; IDO-1; INDO; IDO; Indoleamine-Pyrrole 2,3 Dioxygenase; Indolamine 2,3 Dioxygenase; Indole 2,3-Dioxygenase; EC 1.13.11.52; IDO1

Function:

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.IDO1 (Indoleamine 2,3-Dioxygenase 1) is a Protein Coding gene. Diseases associated with IDO1 include Listeriosis and Trypanosomiasis. Among its related pathways are Histidine, lysine, phenylalanine, tyrosine, proline and tryptophan catabolism and superpathway of tryptophan utilization. Gene Ontology (GO) annotations related to this gene include heme binding and tryptophan 2,3-dioxygenase activity.Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:23103127, PubMed:25157255, PubMed:14502282, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885).

Chromosomal Localization:

8p11.21

Expression System:

E. Coli

Accession Number:

BC027882.1

UniProt Accession Number:

P14902

DNA Source:

OB : MHS1010-7430088

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

51130

Buffers:

PBS

Expressed Sequence:

SNAMAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLP
DLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHG
DVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYEN
MDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLL
KALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGL
VYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRR
YMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHL
QIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEK
SLLKEG

Native Sequence:

MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLI
ESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVR
KVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDV
LFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKAL
LEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE
GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMP
PAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIV
TKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLL
KEG

Calculated Isoelectric Point:

6.87

Molecular Weight:

45.33

Last Updated:

09/02/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of IDO1 (rAg 10898) with molecular weight standards in lane 1
Click image to enlarge

CPTC-IDO1 PAGE

PAGE of IDO1 (rAg 10898) with molecular weight standards in lane 1

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SOPs

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