Indoleamine-pyrrole 2,3 dioxygenase


Background

Catalog Number:

CPTC-IDO1-1

Target Antigen:

Indoleamine-pyrrole 2,3 dioxygenase

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

11/14/2019

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC NCI60

CPTC-IDO1-1 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  IHC Tissue

CPTC-IDO1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-IDO1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-IDO1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-IDO1-1 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-IDO1-1 Western Blot

Result: Positive

Western Blot using CPTC-IDO1-1 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Background

Catalog Number:

CPTC-IDO1-2

Target Antigen:

Indoleamine-pyrrole 2,3 dioxygenase

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

11/14/2019

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC NCI60

CPTC-IDO1-2 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-IDO1-2. Titer: 1:8000 Click image to enlarge

CPTC-IDO1-2 IHC Tissue

Result: Negative

Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-IDO1-2. Titer: 1:8000


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-IDO1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-IDO1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-IDO1-2 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-IDO1-2 Western Blot

Result: Positive

Western Blot using CPTC-IDO1-2 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Background

NCI Identification Number:

10898

Antigen Name:

Indoleamine-pyrrole 2,3 dioxygenase

CPTC Name:

CPTC-IDO1

Keywords:

CD107B; EC 1.13.11.52; IDO; INDO

Function:

Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.52). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.

Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.

Chromosomal Localization:

8p12 - p11

Expression System:

E. Coli

NCBI Accession Number:

BC027882.1

Swiss Prot Accession Number:

P14902

DNA Source:

OB : MHS1010-7430088

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

51130

Buffers:

PBS

Expressed Sequence:

SNAMAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLP
DLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHG
DVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYEN
MDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLL
KALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGL
VYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRR
YMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHL
QIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEK
SLLKEG

Native Sequence:

MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLI
ESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVR
KVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDV
LFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKAL
LEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE
GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMP
PAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIV
TKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLL
KEG

Calculated Isoelectric Point:

6.87

Molecular Weight:

45.33

Last Updated:

10/10/2019

Links

Characterization Data

Gel

Click to enlarge image PAGE of IDO1 (rAg 10898) with molecular weight standards in lane 1
Click image to enlarge

CPTC-IDO1 PAGE

PAGE of IDO1 (rAg 10898) with molecular weight standards in lane 1

SOPs

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