Indoleamine-pyrrole 2,3 dioxygenase


Background

Catalog Number:

CPTC-IDO1-1

Target Antigen:

Indoleamine-pyrrole 2,3 dioxygenase

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

03/05/2015

Antigen Recognition(s):

recombinant full-length

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Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-IDO1-1 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-IDO1-1 Western Blot

Western Blot using CPTC-IDO1-1 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-IDO1-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

  NCI 60 Protein Array

CPTC-IDO1-1 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

  IHC NCI60

CPTC-IDO1-1 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example
Close

IHC Cell Line Images

IHC NCI60 Cell Line Array example
  IHC Tissue

CPTC-IDO1-1 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Background

Catalog Number:

CPTC-IDO1-2

Target Antigen:

Indoleamine-pyrrole 2,3 dioxygenase

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

06/13/2017

Antigen Recognition(s):

recombinant full-length

Purchase
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-IDO1-2 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-IDO1-2 Western Blot

Western Blot using CPTC-IDO1-2 as primary Ab against IDO1 (Ag 10898) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-IDO1-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

  NCI 60 Protein Array

CPTC-IDO1-2 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

  IHC NCI60

CPTC-IDO1-2 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example
Close

IHC Cell Line Images

IHC NCI60 Cell Line Array example
  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-IDO1-2. Titer: 1:8000 Click image to enlarge

CPTC-IDO1-2 IHC Tissue

Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-IDO1-2. Titer: 1:8000

Background

NCI Identification Number:

10898

Antigen Name:

Indoleamine-pyrrole 2,3 dioxygenase

CPTC Name:

CPTC-IDO1

Keywords:

CD107B; EC 1.13.11.52; IDO; INDO

Function:

Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.52). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.

Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.

Chromosomal Localization:

8p12 - p11

Expression System:

E. Coli

NCBI Accession Number:

BC027882.1

Swiss Prot Accession Number:

P14902

DNA Source:

OB : MHS1010-7430088

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

51130

Buffers:

PBS

Expressed Sequence:

SNAMAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLP
DLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHG
DVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYEN
MDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLL
KALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGL
VYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRR
YMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHL
QIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEK
SLLKEG

Native Sequence:

MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLI
ESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVR
KVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDV
LFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKAL
LEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE
GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMP
PAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIV
TKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLL
KEG

Calculated Isoelectric Point:

6.87

Molecular Weight:

45.33

Last Updated:

11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-IDO1-2. Titer: 1:8000
Click image to enlarge

CPTC-IDO1 PAGE

PAGE of IDO1 (rAg 10898) with molecular weight standards in lane 1

SOPs

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