Background
Catalog Number:
CPTC-HUS1-1
Target Antigen:
HUS1 Checkpoint Clamp Component Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
09/05/2024
Antigen Recognition(s):
Peptide, Endogenous
External Links
- Human Protein Atlas CPTC-HUS1-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (482.1 KB)
CPTC-HUS1-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immunofluorescence - HPA
CPTC-HUS1-1 evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to nucleoplasm.
Human assay: THP-1 fixed with PFA, dilution: 1:200
Human assay: U2OS fixed with PFA, dilution: 1:200
Characterization SOP Files
Background
NCI Identification Number:
00486
Antigen Name:
HUS1 Checkpoint Clamp Component Peptide 1
CPTC Name:
CPTC-HUS1 Peptide 1
Aliases:
HUS1 Checkpoint Clamp Component; Checkpoint Protein HUS1; Hus1+-Like Protein; HHUS1; HUS1 (S. Pombe) Checkpoint Homolog; HUS1 Checkpoint Homolog (S. Pombe); HUS1 Checkpoint Homolog; HUS1
Function:
The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.HUS1 (HUS1 Checkpoint Clamp Component) is a Protein Coding gene. Diseases associated with HUS1 include Fanconi Anemia, Complementation Group G and Xeroderma Pigmentosum, Variant Type. Among its related pathways are DNA Double-Strand Break Repair and Cell Cycle, Mitotic. An important paralog of this gene is HUS1B.Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.
Chromosomal Localization:
7p12.3
Accession Number:
NP_004498.1
UniProt Accession Number:
O60921
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
IVTHDIPIK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
990
Last Updated:
04/30/2021
Links
- EnsEMBL - EnsEMBL HUS1
- Entrez - Entrez HUS1
- Uniprot - Uniprot O60921
Characterization Data
SOPs
No SOPs available.
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