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Heat Shock 27kDa Protein 1 Peptide 2

Antibody: CPTC-HSPB1-5
Background

Catalog Number:

CPTC-HSPB1-5

Target Antigen:

Heat Shock 27kDa Protein 1 Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (409.1 KB)

CPTC-HSPB1-5 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-HSPB1-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-703) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma. Click image to enlarge

CPTC-HSPB1-5 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-HSPB1-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-703)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma.


Characterization SOP Files

  Immunofluorescence

CPTC-HSPB1-5 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF7 cells using CPTC-HSPB1-5 antibody. HSPB1 protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (409.1 KB)

CPTC-HSPB1-5 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-HSPB1-5 as primary Ab against full-length recombinant Ag 10175 (lane 2). Also included are molecular wt. standards (lane 1) and the SNCG-3 Ab as positive control (lane 3). Click image to enlarge

CPTC-HSPB1-5 NCI60 Western blot

Result: Positive

Western Blot using CPTC-HSPB1-5 as primary Ab against full-length recombinant Ag 10175 (lane 2). Also included are molecular wt. standards (lane 1) and the SNCG-3 Ab as positive control (lane 3).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-HSPB1-5 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-HSPB1-5 NCI60 Western blot

Result: Positive

Western Blot using CPTC-HSPB1-5 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: Heat Shock 27kDa Protein 1 Peptide 2
Background

NCI Identification Number:

00030

Antigen Name:

Heat Shock 27kDa Protein 1 Peptide 2

CPTC Name:

CPTC-HSPB1 Peptide 2

Aliases:

HSPB1; CMT2F; HMN2B; DKFZp586P1322; HS.76067; HSP27; HSP28; Hsp25; HspB1; OTTHUMP00000024846; SRP27

Function:

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Involved in stress resistance and actin organization

Chromosomal Localization:

7q11.23

Accession Number:

NM_001540

UniProt Accession Number:

P04792

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

VSLDVNHFAPDELTVK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1760

Last Updated:

06/11/2013

Links

Characterization Data

SOPs

No SOPs available.

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